Summary
We have examined several gene expressions during the process of compensatory renal growth in mice following unilateral nephrectomy. On the 3rd, 5th and 7th days after operation in young mice (age: 5w), unilateral nephrectomy induced weight gain of the remaining kidney, but not in adults (age: 15w). It also induced maximum expression of c-H-ras and c-K-ras on the 3rd day in both young and adult mice, but there was no increase in N-ras in either. The expression levels of c-H-ras and c-K-ras were higher in young mice than in adults. However no expression of c-myc was detected at any point. Expression of metallothionein-I (MT-I) gene was detected during the first 12 h after unilateral nephrectomy both in the liver and the contralateral kidney. These data suggest that c-H-ras and c-K-ras gene expressions are in some way related to compensatory renal growth in mice and may be strongly related to hyperplasia in the contralateral kidney.
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References
Austin III H, Goldin H, Preuss HG (1981) Humoral regulation of growth. Nephron 27:163
Beer DG, Zweifel KA, Simpson DP, Pitot HC (1987) Specific gene expression during comepensatory renal hypertrophy in the rat. J Cell Physiol 131:29
Bishop JM (1983) Cellular oncogenes and retroviruses. Ann Rev Biochem 52:301
Bucher NLR (1967) Experimental aspects of hepatic regeneration. N Eng J Med 277:686 and 738
Carl-Henrik H, Bengt W (1984) Growth factors: mechanism of action and relation to oncogenes. Cell 37:9
Cooper GM (1982) Cellular transforming genes. Science 218:801
Durnam DM, Hoffman JS, Quaife CJ, Benditt EP, Chen HY, Brinster RL, Palmiter RD (1984) Induction of mouse metallothionein-I mRNA by bacterial endotoxin is independent of metals and glucocorticoid hormones. Proc Natl Acad Sci USA 81:1053
Fine LG (1986) The biology of renal hypertrophy. Kidney Int 29:619
Goyette M, Petropoulos CJ, Shank PR, Fausto N (1984) Regulated transcription of c-Ki-ras and c-myc during compensatory growth of rat liver. Mol Cell Biol 4:1493
John MC, Alan EP, Raymond JM, William JR (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 19:5294
Karp R, Brasel JA, Winick M (1971) Compensatory kidney growth after uninephrectomy in adult and infant rats. Am J Dis Child 121:186
Kaufman JM, Hardy R, Hayslett JP (1975) Age-dependent characteristics of compensatory renal growth. Kidney Int 8:21
Lu E-X, Wu C-P, Gu F-L (1989) Age factor in post-nephrectomy compensatory renal growth. Urol Res 17:135
Makino R, Hayashi K, Sugimura T (1984) c-myc transcript is induced in rat liver at a very early stage of regeneration or by cyclohemixide treatment. Nature 310:697
Norman J, Badie-Dezfooly B, Nord EP, Schlosser J, Chaudhari A, Fine LG (1987) EGF-induced mitogenesis in proximal tubular cells: potenciation by angiotensin II. Am J Physiol 253:F299
Norman LT, Bohman RE, Fischmann G, Bowen JW, McDonough A, Slamon D, Fine LG (1988) Pattern of mRNA expression during early cell growth differ in kidney epithelial cells destined to undergo compensatory hypertrophy versus regenerative hyperplasia. Proc Natl Acad Sci USA 85:6768
Saphir O (1927) The state of the glomerulus in experimental hypertrophy of the kidney of rabbits. Am J Pathol 3:329
Yamamoto N, Kanetake H, Yamada J (1983) In vitro evidence from tissue cultures to prove the existence of rabbit and human renotropic growth factor. Kidney Int 23:616
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Nomata, K., Igarashi, H., Kanetake, H. et al. Expression of ras gene family as result of compensatory renal growth in mice. Urol. Res. 18, 251–254 (1990). https://doi.org/10.1007/BF00294767
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DOI: https://doi.org/10.1007/BF00294767