Skip to main content
SpringerLink
Log in

Automated image analysis DNA cytometry in testicular cancer

  • Original Paper
  • Published:
Urological Research Aims and scope Submit manuscript

Abstract

The value of automated DNA cytometry for differentiation of testis cancer was evaluated in 54 seminomas, 13 HCG-positive seminomas, and 48 embryonal carcinomas. Slices of paraffin embedded tissue were enzymatically digested and stained with Feulgen SITS after fixation on glass slides. Automated DNA cytometry was performed with a Modular Image Analysis Computer (MIAC). DNA histogram phenotpye and computed DNA indices were correlated with the different tumor types. The ratio of hypertriploid to hypotriploid increased from HCG-positive seminoma over embryonal carcinoma to seminoma. The following mathematical DNA indices were found to correlate with tumor type: mean ploidy, 2c deviation index, 5c exceeding rate, variation coefficient of the GO/1 fraction and DNA nucleus diameter correlation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Allhoff EP, Liedke S, Riese W de, Stief CG, Tanke HJ, Schneider B, Kramer AEJL, Jonas U (1991) Assessment of individual prognosis for patients with NSCGT/CS I. J Urol 145:367A

    Google Scholar 

  2. Atkin NB, Kay R (1979) Prognostic significance of modal DNA value and other factors in malignant tumours, based on 1465 cases. Br J Cancer 40:210

    Google Scholar 

  3. Böcking A (1982) Algorithmus für ein DNS-Malignitäts-Grading. Verh Dtsch Ges Pathol 66:540

    Google Scholar 

  4. Böcking A, Adler CP, Common HH, Hilgarth M, Granzen B, Auffermann W (1984) Algorithm for DNA-cytometric diagnosis and grading of malignancy. Anal Quant Cytol 6:1

    Google Scholar 

  5. Bürger RA, Braun MH, Witzsch U, Potratz D, Stöckle M, Hohenfellner R (1993) Automated DNA-image analysis in testicular cancer. Value of 5c exceeding rate as prognostic factor. J Urol 149:390A

    Google Scholar 

  6. Codesal J, Paniagua R, Regadera J, Fachal C, Nistal M (1991) Significance of DNA quantification in testicular germ cell tumors. Andrologia 23:381

    Google Scholar 

  7. El-Naggar AK, Ro JY, McLemore D, Ayala AG, Batsakis JG (1992) DNA ploidy in testicular germ cell neoplasms. Histogenetic and clinical implications. Am J Surg Pathol 16:611

    Google Scholar 

  8. Fosså SD, Pettersen EO, Thorud E, Melvik JE, Ous S (1985) DNA flow cytometry in human testicular cancer. Cancer Lett 28:55

    Google Scholar 

  9. Fosså SD, Nesland JM, Waehre H, Åmellem O, Pettersen EO (1991) DNA ploidy in the primary tumor from patients with nonseminomatous testicular germ cell tumors clinical stage I. Cancer 40:129

    Google Scholar 

  10. Graff WE de, Oosterhuis JW, Jong B de, Dam A, Putten WLJ van, Castedo SMMJ, Sleijfer DT, Schraffordt Koops H (1992) Ploidy of testicular carcinoma in situ. Lab Invest 66:166

    Google Scholar 

  11. Hiddemann W, Schumann J, Andreef M, Barlogie B, Hermann CJ, Leif RC, Mayall BH, Murphy RF, Sandberg AA (eds) (1984) Convention on Nomenclature for DNA Cytometry. Committee on Nomenclature, Society for Analytical Cytology. Cancer Genet Cytogenet 13: 181

  12. Kleinhans G, Hacker-Klom U, Göhde W, Körner F, Schumann J (1986) Zellkinetische Untersuchungen menschlicher maligner Hodentumoren. Urologe [A] 25:294

    Google Scholar 

  13. Koss LG, Wersto RP, Simmons DA, Deitch D, Herz F, Freed SZ (1989) Predictive value of DNA measurements in bladder washings. Comparison of flow cytometry, image cytophotometry, and cytology in patients with a past history of urethelial tumors. Cancer 64:916

    Google Scholar 

  14. Ljungberg B, Stenling R, Roos G (1986) Prognostic value of deoxyribonucleic acid content in metastatic renal cell carcinoma. J Urol 139:801

    Google Scholar 

  15. Costerhuis JW, Castedo SMMJ, Jong B de, Cornelisse CJ, Dam A, Sleijfer DT, Koops HS (1989) Ploidy of primary germ cell tumors of the testis. Pathogenetic and clinical relevance. Lab Invest 60:14

    Google Scholar 

  16. Otto U, Baisch H, Huland H, Klöppel G (1984) Tumor cell deoxyribonucleic acid content and prognosis in human renal cell cancer. J Urol 132:237

    Google Scholar 

  17. Seppelt U, Sprenger E (1984) Nuclear DNA cytophotometry in prostate carcinoma. Cytometry 5:258

    Google Scholar 

  18. Sledge GW, Eble JN, Roth BJ, Wuhrman BP, Einhorn LH (1987) Flow cytometry derived DNA content of the primary lesions of advanced germ cell tumours. Int J Androl 10:115

    Google Scholar 

  19. Sprenger E, Volk L, Michaelis WE (1974) Die Aussagekraft der Zellkern-DNS-Bestimmung bei der Diagnostik des Prostatakarzinoms. Beitr. Pathol 153:370

    Google Scholar 

  20. Stöckle M, Tanke HJ, Mesker WE, Ploem JS, Jonas U, Hohenfellner R (1987) Automated DNA-image cytometry: a prognostic tool in infiltrating bladder carcinoma. World J Urol 5:127

    Google Scholar 

  21. Stöckle M, Störkel S, Mielke R, Steinbach F, El-Damanhoury H, Voges G, Hohenfellner R (1991) Characterization of conservatively resected renal tumors using automated image analysis DNA cytometry. Cancer 68:1926

    Google Scholar 

  22. Winkler HZ, Rainwater LM, Myers RP, Farrow GM, Therneau TM, Zincke H, Lieber MM (1988) Stage D1 prostatic adenocarcinoma: significance of nuclear DNA ploidy patterns studied by flow cytometry. Mayo Clin Proc 63:103

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Urologische Klinik, Johannes-Gutenberg-Universität, Langenbeckstrasse 1, D-55131, Mainz, Germany

    R. A. Bürger, U. Witzsch, H. Engel & M. Stöckle

  2. Klinik für Pathologie, Johannes-Gutenberg-Universität, Langenbeckstrasse 1, D-55131, Mainz, Germany

    D. Potratz

Authors
  1. R. A. Bürger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. U. Witzsch
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Engel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Stöckle
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D. Potratz
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bürger, R.A., Witzsch, U., Engel, H. et al. Automated image analysis DNA cytometry in testicular cancer. Urol. Res. 22, 17–20 (1994). https://doi.org/10.1007/BF00431543

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00431543

Key words

Search

Navigation