Abstract
Concentrations of α and β-subunits of S100 protein (S100-α and S100-β) in rat kidney neoplasms, including renal cell and mesenchymal tumors, were determined using a highly sensitive enzyme immunoassay, and both types immunohistochemically localized in tissue sections. Concentration of S100-α in each histological type of rat tumor were lower than in normal kidney, whereas levels of S100-β (mean±SE: 29.7±14.2 ng/mg protein, n=15) in renal cell tumors were significantly higher than in normal kidneys (0.55±0.06 ng/mg protein, n=7), or mesenchymal tumors (1.21±0.43 ng/mg protein, n=9). In normal rat kidney tissues S100-α was immunohistochemically positive in epithelial cells of the distal tubules, the thin limbs of loops of Henle, and the collecting ducts. No appreciable immunostaining for S100-β was found in any nephron segment. Both S100-α and S100-β were positive for renal cell tumors, indicating new appearance of the latter during renal carcinogenesis in rats.
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Bannasch P, Zerban H (1990) Animal models and renal carcinogenesis. In: Eble JN (ed) Tumors and tumor-like conditions of the kidneys and ureters. Churchill Livingstone, New York, p 1
Bennington JL, Beckwith JB (1975) Renal adenocarcinoma. In: Firminger HI (ed) Tumors of the kidney, renal pelvis, and ureter, fascicle 12. Armed Forces Institute of Pathology, Washington DC, p 93
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248
Donato R, Isobe T, Okuyama T (1985) S-100 proteins and microtubules: analysis of the effects of rat brain S-100 (S-100b) and ox brain S-100a0, S-100a and S-100b on microtubule assembly-disassembly. FEBS Lett 186:65
Fanò G, Fulle S, Torre GD, Giambanco I, Aisa MC, Donato R, Calissano P (1988) S-100b protein regulates the activity of skeletal muscle adenylate cyclase in vitro. FEBS Lett 240:177
Haimoto H, Hosoda S, Kato K (1987) Differential distribution of immunoreactive S100-α and S100-β proteins in normal nonnervous human tissues. Lab Invest 57:489
Ishiguro Y, Kato K, Ito T, Nagaya M (1983) Determination of three enolase isozymes and S-100 protein in various tumors in children. Cancer Res 43:6080
Ishikawa H, Nogami H, Shirasawa N (1983) Novel clonal strains from adult rat anterior pituitary producing S-100 protein. Nature 303:711
Isobe T, Okuyama T (1981) The amino-acid sequence of the α subunit in bovine brain S-100a protein. Eur J Biochem 116:79
Isobe T, Ishioka N, Masuda T, Takahashi Y, Ganno S, Okuyama T (1983) A rapid separation of S100 subunits by high performance liquid chromatography: the subunit compositions of S100 proteins. Biochem Int 6:419
Ito N (1973) Experimental studies on tumors of the urinary system of rats induced by chemical carcinogens. Acta Pathol Jpn 23:87
Kato K, Kimura S (1985) S100a0 (αα) protein is mainly located in the heart and striated muscles. Biochim Biophys Acta 842:146
Kato K, Haimoto H, Ariyoshi Y, Horisawa M, Washida H, Kimura S (1985) High levels of S-100a0 (αα) protein in tumor tissues and in sera of patients with renal cell carcinoma. Jpn J Cancer Res 76:856
Kato K, Haimoto H, Shimizu A, Tanaka J (1987) Enzyme immunoassay for measurement of the α subunit of S100 protein in human biological fluids. Biomed Res 8:119
Kato K, Suzuki F, Ogasawara N (1988) Induction of S100 protein in 3T3-L1 cells during differentiation to adipocytes and its liberating by lipolytic hormones. Eur J Biochem 177:461
Kimura S, Kato K, Semba R, Isobe T (1984) Regional distribution of S-100a0 (αα), S-100a (αβ), and S-100b (ββ) in the bovine central nervous tissue determined with a sensitive enzyme immunoassay system. Neurochem Int 6:513
Kligman D, Marshak DR (1985) Purification and characterization of a neurite extension factor from bovine brain. Proc Natl Acad Sci USA 82:7136
McGiven AR, Ireton HJC (1972) Renal epithelial dysplasia and neoplasia in rats given dimethylnitrosamine. J Pathol 108:187
Molin S-O, Rosengren L, Baudier J, Hamberger A, Haglid K (1985) S-100 alpha-like immunoreactivity in tubules of rat kidney: a clue to the function of a “brain-specific” protein. J Histochem Cytochem 33:367
Moore BW (1965) A soluble protein characteristic of the nervous system. Biochem Biophys Res Commun 19:739
Nakano Y (1991) Induction of renal pelvic tumors in rats by N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) and uracil. Nagoya J Med Sci 53:65
Patel J, Marangos PJ, Heydorn WE, Chang G, Verma A, Jacobowitz D (1983) S-100-mediated inhibition of brain protein phosphorylation. J Neurochem 41:1040
Semba R, Kato K, Isobe T, Kashiwamata S (1987) Purification of S-100a0 protein from rat kidney. Brain Res 401:9
Suzuki F, Kato K, Nakajima T (1984) Hormonal regulation of adiposc S-100 protein release. J Neurochem 43:1336
Takashi M, Haimoto H, Murase T, Mitsuya H, Kato K (1988) An immunochemical and immunohistochemical study of S100 protein in renal cell carcinoma. Cancer 61:889
Takashi M, Zhu Y, Hasegawa S, Miyake K, Takagi Y, Kato K (1990) Evaluation of the alpha subunit of S100 protein in sera as a tumor marker for renal cell carcinoma. Jpn J Clin Urol 44:1055
Tsuda H, Sakata T, Masui T, Imaida K, Ito N (1984) Modifying effects of butylated hydroxyanisole, ethoxyquin and acetaminophen on induction of neoplastic lesions in rat liver and kidney initiated by N-ethyl-N-hydroxyethylnitrosamine. Carcinogenesis 5:525
Zimmer DB, Van Eldik LJ (1986) Identification of a molecular target for the calcium-modulated protein S100: fructose-1,6-bisphosphate aldolase. J Biol Chem 261:11424
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Takashi, M., Sakata, T., Nakano, Y. et al. Elevated concentrations of the β-subunit of S100 protein in renal cell tumors in rats. Urol. Res. 22, 251–255 (1994). https://doi.org/10.1007/BF00541902
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DOI: https://doi.org/10.1007/BF00541902