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Allopurinol blocks shock-wave-induced rises in cytosolic calcium levels in MDCK cells

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Abstract

Allopurinol has been reported to ameliorate the side effects in patients following shock wave lithotripsy (SWL); however, the mechanism has not been studied. We have examined the protective effect of allopurinol on Madin-Darby canine kidney (MDCK) cells after shock wave exposure (SWE) by determining the release of aspartate aminotransferase (ASAT) and lactate dehydrogenase (LD), and the resting cytosolic Ca2+ concentration ([Ca2+]i). In SWE-treated cells, the release of ASAT and LD increased immediately, but largely transiently, by approximately 23% and 5-fold over control, respectively. Within 1–6 h after SWE there was a gradual rise in the resting [Ca2+]i of 16–137% above control. Allopurinol did not affect the transient enzyme release but blocked the long-term rises in the resting [Ca2+]i. The transient changes in [Ca2+]i evoked by two hormones, ATP and bradykinin, and a drug that releases Ca2+ from internal Ca2+ stores, thapsigargin, were only slightly affected in allopurinol-treated cells. We conclude that the protection conferred by allopurinol on patients treated with SWL might involve a direct protection of the kidney cells by maintaining a normal resting [Ca2+]i.

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References

  1. Assimos DG, Boyce WH, Furr EG, Espeland NA, Holmes RP, Harrision LH, Kroovand RL, McCullough DL (1989) Selective elevation of urinary enzyme levels after extracorporeal shock wave lithotripsy. J Urol 142:687

    Google Scholar 

  2. Baumgartner BR, Dickey KW, Ambrose SS (1987) Kidney changes after extracorporeal shock wave lithotripsy. Radiology 163:531

    Google Scholar 

  3. Chaussy C, Brendel W, Schmiedt E (1980) Extracorporeally-induced destruction of kidney stones by shock waves. Lancet 2:1265

    Google Scholar 

  4. Cheung JY, Constantine JM, Bonventre JV (1986) Regulation of cytosolic free calcium concentration in cultured renal epithelial cells. Am J Physiol 251:F690

    Google Scholar 

  5. Corbally MT, Ryan J, Fitzpatrick J, Fitzgerald RJ (1991) Renal function following extracorporeal lithotripsy in children. J Pediatr Surg 26:539

    Google Scholar 

  6. Doctor RB, Mandel LJ (1991) Minimal role of xanthine oxidase and oxygen free radicals in rat renal tubular reoxygenation injury. J Am Soc Nephrol 1:959

    Google Scholar 

  7. Emmerson BT (1984) Therapeutics of hyperuricemia and gout. Med J Aust 141:31

    Google Scholar 

  8. Gaush CR, Hard WL, Smith TF (1966) Characterization of an established line of canine kidney cells. Proc Soc Exp Biol Med 122:931

    Google Scholar 

  9. Gilbert BR, Riehle RA, Vaughan ED Jr (1988) Extracorporeal shock wave lithotripsy and its effects on renal function. J Urol 139:482

    Google Scholar 

  10. Grynkiewicz G, Poepie M, Tsien RY (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem 260:3440

    Google Scholar 

  11. Humes HD (1986) Role of calcium in pathogenesis of acute renal failure. Am J Physiol 250:F579

    Google Scholar 

  12. Kishimoto T, Yamamoto K, Sugimoto T, Yashihara H, Maekawa M (1986) Side effects of extracorporeal shock-wave exposure in patients treated by extracorporeal shock-wave lithotripsy for upper urinary tract stones. Eur Urol 12:308

    Google Scholar 

  13. Li B, Zhou W, Li P (1995) Protective effects of nifedipine and allopurinol on high energy shock wave induced acute changes of renal function. J Urol 153:596

    Google Scholar 

  14. Lingeman JE, Woods JR, Toto PD (1990) Blood pressure changes following extracorporeal shock wave lithotripsy and other forms of treatment for nephrolithiasis. JAMA 263:1789

    Google Scholar 

  15. Marotto ME, Thurman RG, Lemasters JJ (1988) Early midzonal cell death during low-flow hypoxia in the isolated, perfused rat liver: protection by allopurinol. Hepatology 8:585

    Google Scholar 

  16. Mccord JM (1985) Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med 312:159

    Google Scholar 

  17. Mccord JM, Roy RS (1982) The pathophysiology of superoxide: roles in inflammation and ischemia. Can J Physiol Pharmacol 60:1346

    Google Scholar 

  18. Morgan TR, Laudone VP, Heston WD, Zeitz L, Fair WR (1988) Free radical production by high energy shock waves: comparison with ionizing irradiation. J Urol 139:186

    Google Scholar 

  19. Paulmichl M, Lang F (1988) Enhancement of intracellular calcium concentration by extracellular ATP and UTP in Madin-Darby canine kidney cells. Biochem Bioph Res Co 156:1139

    Google Scholar 

  20. Sakamoto W, Kishimoto T, Nakatani T, Ameno Y, Ohyama A, Kamizuru M, Yasumoto R, Maekawa M (1991) Examination of aggravating factors of urinary excretion ofN-acetyl-β-d-glucosaminidase after extracorporeal shock wave lithotripsy. Nephron 58:205

    Google Scholar 

  21. Strohmaier WL, Bichler KH, Kleinknecht S, Pedro M, Wilber DM (1990) Damaging effects of high energy shock waves on cultured Madin Darby canine kidney (MDCK) cells. Urol Res 18:255

    Google Scholar 

  22. Strohmaier WL, Pedro M, Wilbert DM, Bichler KH (1991) Reduction of shock wave-induced tubular alteration by fosfomycin. J Endourol 5:57

    Google Scholar 

  23. Suhr D, Brummer F, Hulser DF (1991) Cavitation-generated free radicals during shock wave exposure: investigations with cell-free solutions and suspended cells. Ultrasound Med Biol 17:761

    Google Scholar 

  24. Suhr D, Brummer F, Irmer U, Schlachter M, Hulser DF (1994) Reduced cavitation-induced cellular damage by the antioxidative effect of vitamin E. Ultrasonics 32:301

    Google Scholar 

  25. Thastrup O, Cullen PT, Drobak BK, Hanley MR, Dawson AP (1990) Thapsigargin, a tumor promotor, discharges intracellular calcium stores by specific inhibition of the endoplasmic reticulum calcium ATPase. Proc Natl Acad Sci USA 87:2466

    Google Scholar 

  26. Todd JC, Mollitt DL (1995) Leukocyte modulation inhibits endotoxin-induced disruption of intracellular calcium homeostasis. J Trauma 39:1148

    Google Scholar 

  27. Trinchieri A, Zanetti G, Tombolini P, Mandressi A, Ruoppolo M, Tura, Montanari E, Pisani E (1990) Urinary NAG excretion after anesthesia-free extracorporeal lithotripsy of renal stones: a marker of early tubular damage. Urol Res 18:259

    Google Scholar 

  28. Aboolian A, Molen MV, Nord EP (1989) Differential effects of phorbol esters on PGE2 and bradykinin-induced elevation of [Ca2+]i in MDCK cells. Am J Physiol 256:F1135

    Google Scholar 

  29. Wiegmann TB, Welling LW, Beatty DM, Howard DE, Vamos S, Morris SJ (1993) Simultaneous imaging of intracellular [Ca2+]i and pH in single MDCK and glomerular epithelial cells. Am J Physiol 265:C1184

    Google Scholar 

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Author notes

  1. W. -C. Chen

    Present address: Ping Tung Christian Hospital, Ping Tung, Taiwan, Republic of China

Authors and Affiliations

  1. Department of Medical Education and Research, Veterans General Hospital-Kaohsiung, 386 Ta Chung 1st Rd, 813, Kaohsiung, Taiwan, Republic of China

    C. -R. Jan, J. -K. Huang, H. -C. Ou & C. -J. Tseng

  2. Division of Urology, Department of Surgery, Veterans General, Hospital-Kaohsiung, 813, Taiwan, Republic of China

    W. -C. Chen, Y. -H. Lee & J. -K. Huang

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  1. C. -R. Jan
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Correspondence to C. -R. Jan.

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Jan, C.R., Chen, W.C., Lee, Y.H. et al. Allopurinol blocks shock-wave-induced rises in cytosolic calcium levels in MDCK cells. Urol. Res. 25, 427–432 (1997). https://doi.org/10.1007/BF01268861

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  • DOI: https://doi.org/10.1007/BF01268861

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