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A method for determining the dielectric constant and the conductivity of membrane-bounded particles of biological relevance

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Abstract

Numerical assessment is made regarding Pauly and Schwan's theory which describes the dielectric behaviour of a suspension of “shell spheres” as a model of biological membrane-bounded particles. The results indicate that approximate expressions of the theory may give rise to serious errors when applied to particles smaller than about 1 Μm in diameter. With a view to performing analysis according to a general expression of the theory, some of the characteristic responses of dielectric parameters upon changes in phase parameters are examined with particular reference to some numerical ranges of biological interest. On this basis a simplified and systematic procedure is proposed for estimating the phase parameters of particles whose shell phase can be regarded as non-conductive. As the application of the procedure proposed, a set of dielectric data of a synaptosome suspension is analyzed, so that the following three phase parameters are successfully determined: membrane capacitance (or shell phase dielectric constant), internal phase conductivity and internal phase dielectric constant. Some limitations of the procedure are discussed for the cases of conducting shells and small particles.

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Authors and Affiliations

  1. Institute for Chemical Research and Department of Physiology, Kyoto University, Japan

    T. Hanai, N. Koizumi & A. Irimajiri

  2. Institute for Chemical Research, Kyoto University, Uji, 611, Kyoto, Japan

    T. Hanai & N. Koizumi

  3. Department of Physiology, Kyoto University School of Medicine, 606, Kyoto, Japan

    A. Irimajiri

Authors
  1. T. Hanai
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  2. N. Koizumi
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  3. A. Irimajiri
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Hanai, T., Koizumi, N. & Irimajiri, A. A method for determining the dielectric constant and the conductivity of membrane-bounded particles of biological relevance. Biophys. Struct. Mechanism 1, 285–294 (1975). https://doi.org/10.1007/BF00537642

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  • DOI: https://doi.org/10.1007/BF00537642

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