Abstract
Nodularin (NODLN), a cyclic pentapeptide hepatotoxin from the cyanobacterium Nodularia spumigena, induces pores in bilayers of diphytanoyl lecithin (DPhL) and in locust muscle membrane. NODLN increases the surface pressure of a DPhL monolayer; except when the surface pressure of the monolayer is high when the toxin causes a reduction of this parameter. NODLN pores exhibit many open conductance states; the higher state probabilities increasing when the transmembrane pressure is increased. The results from these studies are discussed in terms of two models for a NODLN pore, a torroidal model and a barrel-stave model. The edge energy of the NODLN pore of 1.4× 10−12 J/m is determined.
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Abbreviations
- NODLN:
-
Nodularin
- MCYST-LR:
-
Microcystin-LR
- ADDA:
-
3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid
- DPhL:
-
diphytanoyl lecithin
References
Benz R, Fröhlich O, Läuger P, Montal M (1975) Electrical capacity of black lipid films and of lipid bilayers made from monolayers. Biochim Biophys Acta 394:323–334
Carmichael WW (1989) Freshwater cyanobacteria (blue-green algae) toxins. In: Ownby CL, Odell GV (eds) Natural toxins. Characterization, pharmacology and therapeutics. Pergamon Press, Oxford, pp 3–16
Corronado R, Latorre R (1983) Phospholipid bilayers made from monolayers on patch-clamp pipettes. Biophys J 43:231–236
Dempster J (1993) Computer analysis of electrophysiological signals. Academic Press, London
Derzhanski A, Petrov AG (1982) Multipole model of the molecular asymmetry in thermotropic and lyotropic liquid crystals. Mol Cryst Liq Cryst 89:339–360
Falconer IR, Yeung DSK (1992) Cytoskeletal changes in hepatocytes induced by Microcystis toxins and their relation to hyperphosphorylation of cell proteins. Chem-Biol Interact 81:181–196
Gorczynska E, Huddie P, Mellor I, Miller BA, Ramsey RL, Usherwood PNR, Vais H (1994) Potassium channels of adult locust muscle. (In preparation)
Harbich W, Helfrich W (1979) Alignment and opening of giant lecithin vesicles by electric field. Z Naturforsch 34a:1063–1065
Honkanen RE, Zwiller J, Moore RE, Daily SL, Khatra BS, Dukelow M, Boynton AL (1990) Characterization of microcystin-LR, a potent inhibitor of type 1 and type 2 A protein phosphatases. J Biol Chem 265:19401–19404
Huddie PL, Ramsey RL, Usherwood PNR (1986) Single potassium channels of adult locust (Schistocerca gregaria) muscle recorded using the giga-ohm seal patch-clamp technique. J Physiol 378:60P
Inouye M (1974) A three-dimensional molecular assembly model of a lipoprotein from the Escherichia coli outer membrane. Proc Natl Acad Sci USA 71:2396–2400
Israelachvili J, Marcelja S, Horn RG (1980) Physical principles of membrane organization. Quart Rev Biophys 13:121–200
Kotai J (1972) Instructions for preparation of modified nutrient solution Z8 for algae. Norwegian Institute for water research B 11/69. Blindern, Oslo
MacKintosh C, Beattie KA, Klumpp S, Cohen P, Codd GA (1990) Cyanobacterial microcystin-LR is a potent and specific inhibitor of protein phosphatases 1 and 2 A from both mammals and higher plants. FEBS Lett 264:187–192
Mellor I, Thomas DH, Sansom MSP (1988) Properties of ion channels formed by Staphylococcus aureus δ-toxin. Biochim Biophys Acta 942:280–294
Mellor I, Usherwood PNR, Codd GA, Petrov AG (1993) Nodularin, a cyclic pentameric peptide, forms ion channels in lipid bilayers. C R Acad Bulg Sci 46:53–55
Opsahl LR, Webb WW (1994 a) Transduction of membrane tension by the ion channel alamethicin. Biophys J 66:71–74
Opsahl LR, Webb WW (1994 b) Lipid-glass adhesion in giga-sealed patch-clamped membranes. Biophys J 66:75–79
Pastushenko VF, Petrov AG (1984) Electromechanical mechanism of pore formation in bilayer lipid membranes. Seventh School Biophys. Membrane Transport, Poland, School Proceedings, Wroclaw, vol 2, pp 69–91
Petrov AG (1988) Generalized lipid asymmetry and instability phenomena in membranes. Ninth School on Biophysics of Membrane Transport. School Proceedings, Wroclaw, Poland, vol 2, pp 67–86
Petrov AG, Derzhanski A (1987) Generalized asymmetry of thermotropic and lyotropic mesogens. Mol Cryst Liq Cryst 151: 303–333
Petrov AG, Mitov MD, Derzhanski A (1980) Edge energy and pore stability in bilayer lipid membranes. In Bata L (ed) Advances in liquid crystal research and applications. Pergamon Press, Oxford, pp 695–737
Petrov AG, Ramsey RL, Codd GA, Usherwood PNR (1991) Modelling mechanosensitivity in membranes: effects of lateral tension on ionic pores in a microcystin toxin-containing membrane. Eur Biophys J 20:17–29
Robinson RA, Stokes RH (1965) Electrolyte solutions, 2nd edn. Butterworths, London, p 462
Sansom MSP (1991) The biophysics of peptide models of ion channels. Prog Biophys Mol Biol 55:139–235
Sansom MSP, Mellor IR (1990) Analysis of the gating of single ion channels using current-voltage surfaces. J theor Biol 114: 213–223
Sugar IP (1989) Stochastic model of electric field-induced membrane pores. In: Neumann E, Sowers AE, Jordan CA (eds) Electroporation and electrofusion in cell biology, Plenum Press, New York London, pp 97–110
Yoshizawa S, Matsushima R, Watanabe MF, Harada K-I, Ichihara A, Carmichael WW, Fujiki H (1990) Inhibition of protein phosphatases by microcystin and nodularin associated with hepatotoxicity. J Cancer Res Clin Oncol 116:609–614
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Correspondence to: A. G. Petrov
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Spassova, M., Mellor, I.R., Petrov, A.G. et al. Pores formed in lipid bilayers and in native membranes by nodularin, a cyanobacterial toxin. Eur Biophys J 24, 69–76 (1995). https://doi.org/10.1007/BF00211401
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DOI: https://doi.org/10.1007/BF00211401