Abstract
An improved magnetic bead microrheometer based on phase contrast microscopy allowing high resolution measurements of local deformations within macromolecular networks is applied to study local viscoelastic properties of cross-linked actin networks. By embedding non-magnetic colloidal beads as probes into the networks, the spatial variation of the strain field within cross-linked actin networks can be mapped. Moreover, the Poisson ratio and shear modulus can be measured locally.
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Schmidt, F.G., Ziemann, F. & Sackmann, E. Shear field mapping in actin networks by using magnetic tweezers. Eur Biophys J 24, 348–353 (1996). https://doi.org/10.1007/BF00180376
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DOI: https://doi.org/10.1007/BF00180376