Abstract
The fluorescence depolarization of 1,6-diphenyl-hexatriene was used to study the dynamic properties of the hydrophobic regions of the lipid envelopes of ortho- and paramyxoviruses as well as of the Rous sarcoma virus and of the membrane lipids of susceptible and nonsusceptible cells.
The systems investigated where active and inactive influenza viruses, and NDV virus acting on chick embryo fibroblasts and Rous sarcoma virus acting on susceptible (C/E) and nonsusceptible (C/B) chicken-cell.
Polarization degrees and mean rotational correlation times of DPH embedded in viral lipids were significantly higher than those of DPH in the cell membranes, due to a higher rigidity of the virus envelopes. When suspensions of labelled viruses and unlabelled cells or unlabelled viruses and labelled cells were mixed, a characteristic change of the fluorescence polarization degrees with time was observed. This behaviour was ascribed to a label transfer from virus to cell membranes or vice versa.
While the rate constants of label transfer from virus to cells and cells to virus were about the same for the penetrating viruses the rate constants of label release from inactive virus to cells were much larger than for the migration in the opposite direction.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Andrich, M. P., Vanderkooi, J. M.: Temperature dependence of 1,6-diphenyl-1,3,5-hexatriene fluorescence in phospholipid artificial membranes. Biochemistry 15, 1257–1261 (1976)
Barenholz, Y., Moore, N. F., Wagner, R. R.: Enveloped viruses as model membrane systems: microviscosity of vesicular stomatitis virus and host cell membranes. Biochemistry 15, 3563–3570 (1976)
Bauer, H.: Virion and tumor cell antigens of C-type RNA tumor viruses. Adv. Cancer Res. 20, 275–341 (1974)
Chen, L. A., Dale, R. E., Roth, S., Brand, L.: Nanosecond time-dependent fluorescence depolarization of diphenylhexatriene in dimyristoyllecithin vesicles and the determination of “microviscosity” J. Biol. Chem. 7, 2163–2177 (1977)
Cogan, U., Shinitzky, M., Weber, G., Nishida, T.: Microviscosity and order in the hydrocarbon region of phospholipidcholesterol dispersions determined with fluorescence probes. Biochemistry 12, 521–528 (1973)
Collard, J. G., DeWildt, A., Inbar, M.: Translocation of a fluorescent lipid probe between contacting cells. FEBS-Lett. 90, 24–28 (1978)
Crittenden, L. B., Stone, H. A., Reamer, R. H., Okazaki, W.: Two loci controlling genetic cellular resistance to avian leukosis-sarcoma viruses. J. Virol. 1, 898–904 (1967)
Dale, R. E., Chen, A. L., Brand, L.: Rotational relaxation of the “microviscosity” probe diphenylhexatriene in paraffin oil and egg lecithin vesicles. J. Biol. Chem. 252, 7500–7511 (1977)
Homma, M., Ohuchi, M.: Trypsin action on the growth of Sendai virus in tissue culture cells. III. Structural difference of Sendai virus grown in eggs and tissue culture cells. J. Virol. 12, 1457–1465 (1973)
Huang, R. T. C.: Labeling of animal cells with fluorescent Dansyl cerebroside. Z. Naturforsch. [C] 31, 737–740 (1976)
Huang, R. T. C.: Transfer of Glycolipid between Membranes of Tissue Culture Cells, using Dansylcerebroside as a Model. Z. Naturforsch. [C] 32, 379–383 (1977)
Johnson, S. M., Nicolau, C.: The distribution of 1,6-diphenyl hexatriene fluorescence in normal human lymphocytes. Biophys. Biochem. Res. Commun. 76, 869–874 (1977)
Klenk, H. D., Choppin, P. W.: Lipids of plasma membranes of monkey and hamster kidney cells and of parainfluenza virions grown in these cells. Virology 38, 255–268 (1969)
Klenk, H.-D., Becht, H., Rott, R.: On the structure of the influenza virus envelope. Virology 47, 579–591 (1972)
Klenk, H. D., Choppin, P. W.: Plasma membrane lipids and parainfluenza virus assembly. Virology 40, 939–947 (1970)
Klenk, H.-D., Rott, R., Orlich, M., Blödorn, J.: Activation of influenza A viruses by trypsin treatment. Virology 68, 426–433 (1975)
Landsberger, F. R., Compans, R. W.: Effect of membrane protein on lipid bilayer structure: A spinlable electron spin resonance study of vesicular stomatitis virus. Biochemistry 15, 2356–2360 (1976)
Landsberger, F. R., Lenard, J., Paxton, J., Compans, R. W.: Spin-label electron spin resonance study of the lipid-containing membrane of influenza virus. Proc. Natl. Acad. Sci. USA 68, 2579–2583 (1971)
Lazarowitz, S. G., Choppin, P. W.: Enhancement of the infectivity of influenza A and B viruses by proteolytic cleavage of the hemagglutinin polypeptides. Virology 68, 440–454 (1975)
Levanon, A., Kohn, A.: Changes in cell membrane microviscosity associated with adsorption of viruses. Differences between fusing and nonfusing viruses. FEBS Lett. 85, 245–248 (1978)
Moore, N. F., Barenholz, Y., Wagner, R. R.: Microviscosity of togavirus membranes studied by fluorescence depolarization: Influence of envelope proteins and the host cell. J. Virology 19, 126–135 (1976)
Moore, N. F., Patzer, E. J., Shaw, M. J., Thompson, T. E., Wagner, R. R.: Interaction of vesicular stomatitis virus with lipid vesicles: Depletion of cholesterol and effect on virion membrane fluidity and infectivity. J. Virol. 27, 320–329 (1978)
Nagai, Y., Klenk, H.-D., Rott, R.: Proteolytic activation of virus specific glycoproteins and its significance for the pathogenicity of Newcastle disease virus. Virology 72, 494–508 (1976)
Perram, J., Reimann, B., Klenk, H.-D., Nicolau, C., Polansyk, O. E.: Early molecular events during the interaction of enveloped riboviruses with cells. II. A kinetic study. Biophys. Struct. Mech. 5, 25–32 (1979)
Peterson, I. A., Rubin, H.: Exchange of phospholipids between cultured chick embryo fibroblasts as observed by autoradiography. Exp. Cell Res. 60, 383–392 (1970)
Roche, A. C., Maget-Dana, R., Obrenovitch, A., Hildenbrand, K., Nicolau, C., Monsigny, M.: Interaction between vesicles containing gangliosides and limulin (limulus polyphemus lectin). Fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene. FEBS Lett. 93, 91–96 (1978)
Scheid, A., Choppin, P. A.: Identification and biological activities of paramayxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity by proteolytic cleavage of an inactve precursor protein of Sendai virus. Virology 57, 475–490 (1974)
Sefton, B. M., Gaffney, B. J.: Effect of the viral proteins on the fluidity of the membrane lipids in sindbis virus. J. Mol. Biol. 90, 343–358 (1974)
Vogt, P. K., Ishizaki, R., Duff, R.: Studies on the relationship among avian tumor viruses. In: Subviral carcinogenesis. Ito, Y. (ed.), pp. 297–310. London, New York: Academic Press 1967
Wahl, P.: Fluorescence in biochemistry. Concepts, Vol. 1. Chen, R. F., Edelhoch, H. (eds.), pp. 1–41. New York: Marcel Dekker 1975
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nicolau, C., Klenk, H.D., Hildenbrand, K. et al. Early molecular events in the interaction of enveloped viruses with cells. Biophys. Struct. Mechanism 5, 11–23 (1979). https://doi.org/10.1007/BF00535769
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00535769