Abstract
Immunity against PCC3 teratocarcinoma cells (129, H-2 b) was induced in allogeneic (C3H, H-2 k) mice by preimmunization with L cells (C3H, H-2 k) expressing cosmid-introduced K b or D b genes, but not with nontransfected L cells. In addition, the growth of PCC3 cells in sublethally irradiated (C3H × B6-H-2 bm1)F1 and (C3H × B6-H-2 bm13 )F1 mice bearing the K bm1 and D bm13 mutations, respectively, was either prevented, stopped, or delayed in comparison with the (C3H × B6)F1 (k × b) mice, which failed to reject the PCC3 cells. The teratocarcinoma line OC15S was exceptional because it reacted specifically with Kb- and Db-specific (but not Ib-specific) alloantisera, and because Kb- and Db-specific antibodies could be absorbed by OC15S cells. The subpopulation of OC15S cells bearing the ECMA-7 antigen characteristic for embryonic carcinoma (EC) cells was isolated by the fluorescence-activated cell sorter and was shown to react specifically with Kb- and Db-specific antisera. These experiments show that teratocarcinoma cells express antigens similar or identical to the K-and D-region products of differentiated cells. The lack of expression of class I antigens is thus neither a condition nor a consequence of the pluripotentiality of the EC cells. The exact nature of the major histocompatibility complex antigens on EC cells has yet to be established using the methods of molecular biology and biochemistry.
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References
Artzt, K. and Jacob, F.: Absence of serologically detectable H-2 on primitive teratocarcinoma cells in culture. Transplantation 17: 632–634, 1974
Avner, P. R., Dove, W. F., Dubois, P., Gaillard, J. A., Guénet, J.-L., Jacob, F., Jakob, H., and Shedlovsky, A.: The genetics of teratocarcinoma transplantation: Tumor formation in allogeneic hosts by the embryonal carcinoma cell lines F9 and PCC3. Immunogenetics 7: 103–115, 1978
Cook, R. G., Jenkins, R. N., Flaherty, L., and Rich, R. R.: The Qa-1 alloamigens. II. Evidence for the expression of two Qa-1 molecules by the Qa-1d genotype and for cross-reactivity between Qa-1 and H-2Kf. J. Immunol. 130: 1293–1299, 1983
Croce, C. M., Linnenbach, A., Huebner, K., Parnes, J. R., Margulies, D. H., Apella, E., and Seidman, J. G.: Control of expression of histocompatibility antigens (H-2) and β 2 microglobuhn in F9 teratocarcinoma stem cells. Proc. Natl. Acad. Sci. U.S.A. 78: 5754–5758, 1981
Figueroa, F., Zaleska-Rutczynska, Z., Kusnierczyk, P., and Klein, J.: Crossreactivity between Qa-1 region and H-2K antigens. Transplantation 35: 391–393, 1983
Iványi, D., Cherry, M., and Démant, P.: Molecular heterogeneity of D-end products detected by anti-H-2.28 sera. III. Reactivity of certain anti-H-2.28 alloantisera with Qa-2 antigen. Immunogenetics 15: 477–484, 1982
Jakob, H., Boon, T., Gaillard, J., Nicolas, J. F., and Jacob, F.: Teratocarcinome de la souris: Isolement, culture et propriétés de cellules à potentialités multiples. Ann. Microbiol. 124 B: 269–282, 1973
Johnson, L. L., Clipson, L. J., Dove, W. F., Feilbach, J., Maher, L. J., and Shedlovsky, A.: Teratocarcinoma transplantation antigens are encoded in the H-2 region. Immunogenetics 18: 137–145, 1983
Kemler, R.: Analysis of mouse embryonic cell differentiation. Fortschr. Zool. 2G: 175–180, 1980
Levine, A. and Teresky, A. K.: Teratocarcinoma transplantation rejection loci: Genetic localization of the Gt-1 locus on chromosome 17 and the expression of alternate alleles. Immunogenetics 13: 405–412, 1981
McBurney, M. W.: Clonal lines of teratocarcinoma cells in vitro: Differentiation and cytogenetic characteristics. J. Cell. Physiol. 89: 441–456, 1976
Morello, D., Daniel, F., Baldacci, P., Cayre, Y., Gachelin, G., and Kourilsky, P.: Absence of significant H-2 and β 2 microglobulin mRNA expression by mouse embryonal carcinoma cells. Nature 296: 260–262, 1982
Morello, D., Gachelin, G., Daniel, F., and Kourilsky, F.: Control of expression of the genes coding for major histocompatibility antigens in embryonal carcinoma cells. In L. M. Silver, G. R., Martin, and S. Strickland (eds.): Teratocarcinoma Stem Cells, pp. 421–427, Cold Spring Harbor Laboratory, New York, 1983
Nairn, R., Yamaga, K., and Nathenson, S. G.: Biochemistry of the gene products from murine MHC mutants. Annu. Rev. Genet. 14: 241–277, 1980
Ostrand-Rosenberg, S. and Cohan, V. L.: H-2 negative teratocarcinoma cells become H-2 positive when passaged in genetically resistant host mice. J. Immunol. 126: 2190–2193, 1981
Oudshoorn-Snoek, M., Démant, P., Mellor, A. L., and Flavell, R. A.: Antigenic characterization of products of cloned H-2 b genes. Transplant. Proc. 15: 2027–2032, 1983
Oudshoorn-Snoek, M., Mellor, A. L., Flavell, R. A., and Démant, P.: A new determinant, Qa-m208, detected on T lymphocytes and transfected L cells by a Kb-specific monoclonal antibody. Immunogenetics 19: 461–474, 1984
Sharrow, S. O., Flaherty, L., and Sachs, D. H.: Serologic cross-reactivity between class I MHC molecules and an H-2-linked differentiation antigen as detected by monoclonal antibodies. J. Exp. Med. 159: 21–40, 1984
Shedlovsky, A., Clipson, L. J., Van de Berg, J. L., and Dove, W. F.: Strong teratocarcinoma transplantation loci, Gt-1 and Gt-2, flank H-2. Immunogenetics 13: 413–419, 1981
Snoek, M., Iványi, D., Nusse, R., and Démant, P.: A new H-2.1-positive D region allele, D dx, controlling two molecules, H-2Ddx and H-2Ldx. Immunogenetics 8: 109–125, 1979
Weiss, E. H., Golden, L., Fahrner, K., Mellor, A. L., Devlin, J. J., Bullman, H., Tiddens, H., Bud, H., and Flavell, R. A.: Organization and evolution of the class I gene family in the major histocompatibility complex of the C57BL/10 mouse. Nature 310: 650–655, 1984
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Démant, P., Oudshoorn-Snoek, M. H-2 class I antigen expression on mouse teratocarcinoma cell lines. Immunogenetics 22, 543–552 (1985). https://doi.org/10.1007/BF00430302
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DOI: https://doi.org/10.1007/BF00430302