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Intermolecular complexes between three human CD1 molecules on normal thymus cells

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Abstract

The first cluster of differentiation (CD1) defines at least three distinct human thymic cell-surface differentiation antigens-CD1a, CD1b, and CD1c. We looked for structural homology of the three CD1 heavy chains at their peptide level by two-dimensional peptide maps. We show here that the CD1a M r 49 000 heavy chain and the CD1b M r 45 000 heavy chain appear to be more homologous to each other than to the CD1c M r 43 000 heavy chain and that only one tyrosil peptide is common to the three heavy chains. Study of the CD1 heavy chains from several individuals reveals a very limited polymorphism of these molecules. We also demonstrate here that CD1a or CD1a-like molecules and other CD1 molecules can form intermolecular complexes on the surface of normal thymus cells. Molecules that are structurally very similar to CD1a molecules are associated noncovalently either with CD1c molecules or with CD1b molecules, and only CD 1a molecules can associate covalently with CD8 molecules. In contrast, we could not find these intermolecular complexes on the surface of leukemic T-cell lines in culture.

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Abbreviations

CD:

cluster of differentiation

mAb:

monoclonal antibody

MHC:

major histocompatibility complex

NP-40:

Nonidet P 40

B2m:

beta-2 microglobulin

PMSF:

phenylmethylsulfonyl fluoride

SDS-PAGE:

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

TL:

thymus leukemia

References

  • Acuto, O., Meuer, S. C., Hodgdon, J. C., Schlossman, S. F., and Reinherz, E. L.: Peptide variability exists within δ and β subunits of the T cell receptor for antigen. J. Exp. Med. 158: 1368–1373, 1983

    Google Scholar 

  • Amiot, M., Bernard, A., Raynal, B., Knapp, W., Deschildre, C., and Boumsell, L.: Heterogeneity of the first cluster of differentiation: characterization and epitopic mapping of three CD1 molecules on normal human thymus cells. J. Immunol. 136: 1752–1758, 1986

    Google Scholar 

  • Amiot, M., Dastot, H., Degos, L., Schmid, M., and Boumsell, L.: Study of the CD1 monoclonal antibodies from the workshop panel. In A. J. McMichael, P. C. L. Beverley, S. Cobbold, M. J. Crumpton, W. Gilks, F. M. Gotch, N. Hogg, M. Horten, N. Ling, I. C. M. MacLennan, D. Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann (eds.): Leucocyte Typing 3, p. 80, Oxford University Press, Oxford, 1987a

    Google Scholar 

  • Amiot, M., Dastot, H., Schmid, M., Bernard, A., and Boumsell, L.: Analysis of CD1 molecules on thymus cells and leukemic T lymphoblasts identifies discrete phenotypes and reveals that CD1 inter molecular complexes are observed only on normal cells. Blood 70: 676–685, 1987b

    Google Scholar 

  • Bernabeu, C., Van de Rijn, M., Lerch, P. J., and Terhorst, C.: β2 microglobulin from serum associates with MHC class I antigens on the surface of cultured cells. Nature 308: 642–645, 1984

    Google Scholar 

  • Boumsell, L. and Bernard, A.: High efficiency of Biozzi's high responder mouse strain in the generation of antibody secreting hybridomas. J. Immunol. Methods 38: 225–229, 1980

    Google Scholar 

  • Boumsell, L., Gelin, C., Coppin, H., Caudan, P., Kalil, J., Pham, D., Raynal, B., and Bernard, A.: Diversity of class I molecule heavy and light chains on human thymus cells. In A. Bernard, L. Boumsell, J. Dausset, C. Milstein, and S. F. Schlossman (eds.): Leucocyte Typing, pp. 257–272, Springer Verlag, Berlin, 1984

    Google Scholar 

  • Boumsell, L., Amiot, M., Raynal, B., Gay Bellile, V., Caillou, B., and Bernard, A.: Epitopic groups of CD1 molecules. In E. L. Reinherz, B. J. Haynes, I. D. Bernstein, and L. M. Nader (eds.): Leucocyte Typing 2, pp. 289–302, Springer Verlag, New York, 1985

    Google Scholar 

  • Buskin, Y., Chorney, M. J., Diamente, E., Fu, S. M., and Wang, C. Y.: Biochemical characterization of the human T6 antigen: a comparison between T6 and murine TL. Mol. Immunol. 21: 821–829, 1984

    Google Scholar 

  • Bushkin, Y., Posnett, D. N., Pernis, B., and Wang, C. Y.: A new HLA-linked T cell membrane molecule related to the chain of the clonotypic receptor is associated with T3. J. Exp. Med. 164: 458–473, 1986

    Google Scholar 

  • Calabi, F. and Milstein, C.: A novel family of human major histocompatibility complex-related genes not mapping to chromosome 6. Nature 323: 540–543, 1986

    Google Scholar 

  • Chorney, M. J., Shen, F. W., Tung, J. S., and Boyse, E. A.: Additional products of the Tla locus of the mouse. Proc. Natl. Acad. Sci. U.S.A. 82: 7044–7047, 1985a

    Google Scholar 

  • Chorney, M. J., Tung, J. S., Bushkin, Y., and Shen, F. W.: Structural characteristics of T1a products. J. Exp. Med. 162: 781–789, 1985b

    Google Scholar 

  • Corte, G., Tonda, P., Cosullich, E., Milstein, C. P., Bargellesi, A., and Ferrarini, M.: Characterization of IgD. I. Isolation of two molecular forms from human serum. Scand. J. Immunol. 9: 141–149, 1979

    Google Scholar 

  • Corte, G., Damiani, G., Calabi, F., Fabbi, M., and Bargellesi, A.: Analysis of HLA-DR polymorphism by two-dimensional peptide mapping. Proc. Natl. Acad. Sci. U.S.A. 78: 534–537, 1981

    Google Scholar 

  • Cotner, T., Mashimo, H., Kung, P. C., Golstein, G., and Strominger, J. L.: Human T cell surface antigens bearing a structural relationship to HLA antigens. Proc. Natl. Acad. Sci. U.S.A. 78: 3858–3862, 1981

    Google Scholar 

  • Due, C., Simonsen, M., and Olsson, L.: The major histocompatibility complex class I heavy chain as a structural subunit of the human cell membrane insulin receptor: implications for the range of biological functions of histocompatibility antigens. Proc. Natl. Acad. Sci. U.S.A. 83: 6007–6011, 1986

    Google Scholar 

  • Elder, J. F., Jensen, F. C., Bryant, M. L., and Lerner, R. A.: Polymorphism of the major envelope glycoprotein (gp70) of routine C-type viruses: virion associated and differentiation antigens encoded by a multi-gene family. Nature 267: 23–28, 1977

    Google Scholar 

  • Feizi, T.: Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are onco-developmental antigens. Nature 314: 53–57, 1985

    Google Scholar 

  • Grasberger, B., Minton, A. P., Delisi, C., and Metzger, H.: Interaction between proteins localized in membranes. Proc. Natl. Acad. Sci. U.S.A. 83: 6258–6262, 1986

    Google Scholar 

  • Hood, L., Kronenberg, M., and Hunkapiller, T.: T cell antigen receptors and the immunoglobulin supergene family. Cell 40: 225–229, 1985

    Google Scholar 

  • IUIS-WHO Nomenclature Subcommittee: Nomenclature for clusters of differentiation (CD) of antigens defined on human leukocyte populations. Bull. WHO 62: 809–811, 1984

    Google Scholar 

  • Kanitakis, J., Schmitt, D., Bernard, A., Boumsell, L., and Thivolet, J.: Anti-D47: a monoclonal antibody reacting with the secretory cells of human eccrine sweat glands. Brit. J. Dermatol. 109: 509–513, 1983

    Google Scholar 

  • Kefford, R. F., Calabi, F., Fearnley, I. M., Burrone, O. R., and Milstein, C.: Serum β2 microglobulin binds to a T cell differentiation antigen and increases its expression. Nature 308: 641–642, 1984

    Google Scholar 

  • Knowles, R. W.: Biochemical analysis of human thymus leukemia like antigens. In A. Bernard, L. Boumsell, J. Dausset, C. Milstein, and S. F. Schlossman (eds.): Leucocyte Typing, pp. 248–272, Springer Verlag, Berlin, 1984

    Google Scholar 

  • Knowles, R. W.: Immunochemical analysis of the T cell specific antigens. In E. L. Reinherz, B. J. Haynes, I. D. Bernstein, and L. M. Nadler (eds.): Leucocyte Typing 2, pp. 259–288, Springer Verlag, New York, 1985

    Google Scholar 

  • Knowles, R. W. and Bodmer, W. F.: A monoclonal antibody recognizing a human TL-like antigen. Eur. J. Immunol. 12: 676–681, 1982

    Google Scholar 

  • Kondo, S., Shimizu, A., Saito, Y., Kinoshita, M., and Honjo, T.: Molecular basis for two different affinity states of the interleukin 2 receptor: affinity conversion model. Proc. Natl. Acad. Sci. U.S.A. 83: 9026–9027, 1986

    Google Scholar 

  • Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685, 1970

    Google Scholar 

  • Ledbetter, J. A., Tsu, T. T., and Clark, E. A.: Covalent association between human thymus leukemia-like antigens and CD8 (Tp32) molecules. J. Immunol. 134: 4250–4254, 1985

    Google Scholar 

  • Lerch, P. G., Van De Rijn, M., Smart, J. E., Knowles, R. W., and Terhorst, C.: Isolation and purification of the human thymocyte antigens T6 and M241. Mol. Immunol. 23: 131–139, 1986

    Google Scholar 

  • Martin, L. H., Calabi, F., and Milstein, C.: Isolation of CD1 genes: a family of major histocompatibility complex-related differentiation antigens. Proc. Natl. Acad. Sci. U.S.A. 83: 9154–9158, 1986

    Google Scholar 

  • McMichael, A. J., Pilch, J. R., Galfre, G., Mason, D. Y., Fabre, J. W., and Milstein, C.: A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. Eur. J. Immunol. 9: 205–210, 1979

    Google Scholar 

  • McMichael, A. J., et al.: Joint report of the Third International Workshop on Human Leucocyte Differentiation Antigen. In A. J. McMichael, P. C. L. Beverley, S. Cobbold, M. J. Crumpton, W. Gilks, F. M. Gotch, N. Hogg, M. Horton, N. Ling, I. C. M. MacLennan, D. Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann (eds.): Leucocyte Typing 3, p. 31, Oxford University Press, Oxford, 1987

    Google Scholar 

  • Milstein, C., Calabi, F., Jarvis, J. M., Kefford, R., Martin, L., and Migone, N.: CD1: a family of MHC-related genes which do not map in chromosome 6. In A. J. McMichael, P. C. L. Beverley, S. Cobbold, M. J. Crumpton, W. Gilks, F. M. Gotch, N. Hogg, M. Horton, N. Ling, I. C. M. MacLennan, D. Y. Mason, C. Milstein, D. Spiegelhalter, and H. Waldmann (eds.): Leucocyte Typing 3, p. 882, Oxford University Press, Oxford, 1987

    Google Scholar 

  • Olive, D., Dubreuil, P., and Mawas, C.: Two distinct TL-like molecular subsets defined by monoclonal antibodies on the surface of human thymocytes with different expression on leukemia lines. Immunogenetics 20: 253–264, 1984

    Google Scholar 

  • Reinherz, E. R., Kung, P. C., Golsten, G., Levey, R. L., and Schlossman, S. F.: Discrete stages of intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T lineage. Proc. Natl. Acad. Sci. U.S.A. 77: 1588–1592, 1980

    Google Scholar 

  • Schneider, C., Newman, R. A., Sutherland, D. R., Asset, U., and Greaves, M. F.: A one step purification of membrane proteins using a high efficiency immunomatrix. J. Biol. Chem. 257: 10766–10769, 1982

    Google Scholar 

  • Snow, P. M., Van De Rijn, M., and Terhorst, C.: Association between the human thymic differentiation antigens T6 and T8. Eur. J. Immunol. 15: 529–532, 1985

    Google Scholar 

  • Takesaki, S., Morrison, S. L., Berger, C. L., Goldstein, G., Chu, A. C., and Edelson, R. L.: Biochemical characterization of a differentiation antigen shared by human epidermal Langerhans cells and cortical thymocytes. J. Clin. Immunol. 3: 1288–1293, 1982

    Google Scholar 

  • Teshigawara, K., Wang, H., Kato, K., and Smith, K. A.: Interleukin 2: high-affinity receptor expression requires two distinct binding proteins. J. Exp. Med. 165: 223–238, 1987

    Google Scholar 

  • Van Agthoven, A. and Terhorst, C.: Further biochemical characterization of the human thymocyte differentiation antigen T6. J. Immunol. 128: 426–432, 1982

    Google Scholar 

  • Van de Rijn, M., Lerch, P. G., Knowles, R. W., and Terhorst, C.: The thymic differentiation markers T6 and M241 are two unusual MHC class I antigens. J. Immunol. 131: 851–855, 1983

    Google Scholar 

  • Van de Rijn, M., Lerch, P. G., Bronstein, B. R., Knowles, R. W., Bhan, A. K., and Terhorst, C.: Human cutaneous dendritic cells express two glycoproteins T6 and M241 which are biochemically identical to those found on cortical thymocytes. Hum. Immunol. 9: 201–210, 1984

    Google Scholar 

  • Williams, A. F.: Immunoglobulin-related domains for cell surface recognition. Nature 314: 579–580, 1985

    Google Scholar 

  • Ziegler, A. and Milstein, C.: A small polypeptide different from β2 microglobulin associated with a human cell surface antigen. Nature 279: 243–244, 1979

    Google Scholar 

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Authors and Affiliations

  1. Hopital Saint-Louis, INSERM U93, Institut de Recherche sur les Maladies du Sang, F-75475, Paris Cedex 10, France

    Martine Amiot, Helene Dastot, Laurent Degos & Laurence Boumsell

  2. Laboratorio di Immunobiologia, Istituti Nazionale per la Ricerca sul canero, Genova, Italy

    Marina Fabbi

  3. Laboratoire d'Immunologie des Tumeurs de l'enfant, Institut Gustave Roussy, F-94805, Villejuif, France

    Alain Bernard

Authors
  1. Martine Amiot
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  2. Helene Dastot
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  3. Marina Fabbi
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  4. Laurent Degos
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  5. Alain Bernard
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  6. Laurence Boumsell
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Amiot, M., Dastot, H., Fabbi, M. et al. Intermolecular complexes between three human CD1 molecules on normal thymus cells. Immunogenetics 27, 187–195 (1988). https://doi.org/10.1007/BF00346585

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  • DOI: https://doi.org/10.1007/BF00346585

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