Abstract
Intra-H-2 recombinant congenic strains are widely used to localize traits to specific subregions of the major histocompatibility complex and have provided evidence for the existence of meiotic recombinational hotspots in mammals. Forty-seven intra-H-2 recombinant strains, including 12 not previously reported, have been identified by serological typing in our laboratory. We have extended the analysis of the cross-over sites in these mice using DNA markers for Ab, Aa, Eb, Ea, Cyp21-ps, D17Tu3, Bat7, and Bat5. The recombinant chromosomes of these congenic strains include loci derived from the a, b, f, k, p, q, r, s, u, and v haplotypes of H-2, providing a diverse panel of strains. Although some alleles of Bat7 could not be distinguished from one another, results from the majority of strains indicated a probable gene order of C4Slp/D17Tu3-Bat7-Bat5-H-2D. No recombinants between Cyp21-ps, C4Slp, and D17Tu3 were observed. The crossover sites in 31 of the 47 intra-H-2 recombinants were within the C4Slp/D17Tu3—H-2D interval; of these 31 crossovers, three were bracketed by D17Tu3 and Bat7, ten by Bat7 and Bat5, seven by Bat5 and H-2D, and 11 by D17Tu3 and Bat5. The results from all 47 strains suggest recombinational hotspots within the C4Slp/D17Tu3—H-2D interval and emphasize the influence that specific haplotypes can have on preferred crossover sites.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bryda, E. C., Depari, J. A., Sant'Angelo, D. B., Murphy, D. B., and Passmore, H. C. Multiple sites of crossing over within the Eb recombinational hotspot in the mouse. Mammalian Genome 2: 123–129, 1992
Carlson, G. A., Goodman, P. A., Lovett, M., Taylor, B. A., Marshall, M., Peterson-Torchia, S. T., Westaway, D., and Prusiner, S. B. Genetics and polymorphism of the mouse prion gene complex: control of scrapie incubation time. Mol Cell Biol 8: 5528–5540, 1988
Chesebro, B., Miyazawa, M., and Britt, W. J. Host genetic control of spontaneous and induced immunity to friend murine retrovirus infection. Annu Rev Immunol 8: 477–499, 1990
Committee for Mouse Chromosome 17: Maps of mouse chromosome 17: first report. Mammalian Genome 1: 5–29, 1991
Crowle, A. J. Micro double diffusion plate tests. In A. J. Crowle (ed.): Immunodiffusion, pp. 286–289, Academic Press, New York, 1973
Demant, P. Corticosteroid-induced cleft palate: Cis interaction of MHC genes and hybrid resistance. Immunogenetics 22: 183–188, 1985
Gasser, D. L., Yadvish, K. N., Trammell, M. A., and Goldman, A. S. Recombinants in the H-2S/H-2D interval of mouse chromosome 17 define the map position of a gene for cleft palate susceptibility. Teratology 38: 571–577, 1988
Hasenkrug, K. J. and Stimpfling, J. H. The antigenic properties of twenty-four new H-2 congenic recombinant mouse strains. Immunogenetics 24: 423–427, 1986
Hasenkrug, K. J., Cory, J. M., and Stimpfling, J. H. Monoclonal antibodies defining mouse tissue antigens encoded by the H-2 region. Immunogenetics 25: 136–139, 1987
Henson, V., Palmer, L., Banks, S., Nadeau, J. H., and Carlson, G. A. Loss of heterozygosity and mitotic linkage maps in the mouse. Proc Natl Acad Sci USA 88: 6486–6490, 1991
Klein, J. Congenic and segregating inbred strains: immunologically important loci. In M. F. Lyon and A. G. Searle (eds.): Genetic Variants and Strains of the Laboratory Mouse, pp. 797–825, Oxford University Press, New York, 1989
Lafuse, W. P., Berg, N., Savarirayan, S., and David, C. S. Mapping of a second recombination hotspot within the I-E region of the mouse H-2 gene complex. J Exp Med 163: 1518–1528, 1986
Lafuse, W. P. and David, C. S. Recombination hotspots within the I region of the mouse H-2 complex map to the Eb and Ea genes. Immunogenetics 24: 352–360, 1986
Lafuse, W. P., Lanning, D., Spies, T., and David, C. S. PFGE mapping and RFLP analysis of the S/D region of the mouse H-2 complex. Immunogenetics 36: 110–116, 1992
Miyazawa, M., Nishio, J., Wehrly, K., and Chesebro, B. Influence of MHC genes on spontaneous recovery from Friend retrovirus-induced leukemia. J Immunol 148: 644–647, 1992
Rembecki, R. M., Kumar, V., David, C. S., and Bennett, M. Bone marrow cell transplants involving intra-H-2 recombinant inbred mouse strains: evidence that hemopoietic histocompatibility-1 (Hh-1) genes are distinct from H-2D or H-2L. J Immunol 141: 2253–2260, 1988
Shiroishi, T., Sagai, T., and Moriwaki, K. A new wild-derived H-2 haplotype enhancing K-IA recombination. Nature 300: 370–372, 1982
Shiroishi, T., Sagai, T., Hanzawa, N., Gotoh, H., and Moriwaki, K. Genetic control of sex-dependent meiotic recombination in the major histocompatibility complex of the mouse. EMBO J 10: 681–686, 1991
Snoek, M., Groot, P. C., Spies, T., Campbell, R. D., and Demant, P. Fine mapping of the crossover-sites in the C4-H-2D region of H-2 recombinant mouse strains. Immunogenetics 34: 409–412, 1991
Spies, T., Bresnahan, M., and Strominger, J. L. Human major histocompatibility complex contains a minimum of 19 genes between the complement cluster and HLA-B. Proc Natl Acad Sci USA 86: 8955–8958, 1989
Steinmetz, M., Minard, K., Horvath, S., McNicholas, J., Srelinger, J., Wake, C., Long, E., Mach, B., and Hood, L. A molecular map of the immune response region from the major histocompatibility complex of the mouse. Nature 300: 35–42, 1982
Steinmetz, M., Stephan, D., and Fischer Lindahl, K. Gene organization and recombinational hotspots in the murine major histocompatibility complex. Cell 44: 895–904, 1986
Stimpfling, J. H. and Reichert, A. E. Strain C57BL/10SgSn and its congenic resistant sublines. Transplantation Proc 2: 29–47, 1970
Teuscher, C., Gasser, D. L., Woodward, S. R., and Hickey, W. F. Experimental allergic orchitis in mice. VI. Recombinations within the H-2S/H-2D interval define the map position of the H-2-associated locus controlling disease susceptibility. Immunogenetics 32: 337–344, 1990
Uematsu, Y., Kiefer, H., Schulze, R., Fischer Lindahl, K., and Steinmetz, M. Molecular characterization of a meiotic recombinational hotspot enhancing homologous equal crossing-over. EMBO J 5: 2123–2129, 1986
Vincek, V., Kawaguchi, H., Mizuno, K., Zaleska-Rutczynska, Z., Kasahara, M. Z., Forejt, J., Figueroa, F., and Klein, J. Linkage map of mouse chromosome 17: localization of 27 new DNA markers. Genomics 5: 773–786, 1989
Wahls, W. P., Wallace, L. J., and Moore, P. D. Hypervariable mini-satellite DNA is a hotspot for homologous recombination in human cells. Cell 60: 95–103, 1990
White, P. C., Chaplin, D. D., Weis, J. H., Dupont, B., New, M. I., and Seidman, J. G. Two steroid 21-hydroxylase genes are located in the murine S region. Nature 312: 465–467, 1984
Womack, J. E. and David, C. S. Mouse gene for neuraminidase activity (Neu-1) maps to the D end of H-2. Immunogenetics 16: 177–180, 1982
Author information
Authors and Affiliations
Additional information
Correspondence to: G. A. Carlson.
Rights and permissions
About this article
Cite this article
Turner, S.K., Miller, C.L., Wettstein, P.J. et al. Meiotic recombination within the H-2K-H-2D interval: characterization of a panel of congenic mice, including 12 new strains, using DNA markers. Immunogenetics 38, 332–340 (1993). https://doi.org/10.1007/BF00210474
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00210474