Abstract
Homozygous typing cells (HTC) were primed, using responding and stimulating lymphocytes of the same HLA-D groups. These intra-HLA-D group primings showed strong specific responses. Restimulation by HLA-D heterozygous and homozygous cell panels showed no correlation between the restimulating determinant and HLA-D. On the other hand, an unrelated individual, not carrying Dw4 and primed to Dw4 HTC, is restimulated by three of four Dw4-HTC. Thus, one non-HLA-D-associated restimulating determinant and another HLA-D-associated determinant could be identified. The differences among the four Dw4 HTC recognized in secondary MLC could reflect either recognition of separate gene products or recognition of separate determinants on the same gene product.
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Bach,.F.H. and van Rood, J.J.: The major histocompatibility complex-genetics and biology.New Engl. J. Med. 295:872–878, 1976
Bach, F., Sondel, P., Sheehy, M., Wank, R., Alter, B., and Bach, M.: The complexity of the HL-A system: a PLT analysis.In F. Kissmeyer-Nielsen (ed.):Histocompatibility Testing 1975, pp. 576. Munksgaard, Copenhagen, 1975
Bradley, B., Sheehy, M., Keuning, J., Termijtelen, A., Franks, D., and van Rood, J.: Phenotyping of HLA-D region products by negative and positive mixed lymphocyte reaction.Immunogenetics 3:573–582, 1976
Dupont, B., Hansen, J.A., and Yunis, E.J.: Human mixed lymphocyte culture reaction: genetics, specificity, and biological implications.Adv. Immunol. 23:108–187, 1976
Dupont, B., Yunis, E.J., Duquesnoy, R., Pollack, M., Oreen, H., Hansen, J.A., Reinsmoen, N., Annen, K., Greenberg, L., Lee, T.D., Whitsett, C., Antonelli, P., and Braun, D.: HLA-D and Ia-like alloantigens in North American Caucasians (U.S. Region 7).In W. Bodmer, R. Batchelor, P. Morris, H. Festenstein, and J. Bodmer (eds.):Histocompatibility Testing 1977; Munksgaard, Copenhagen, in press 1978
Fradelizi, D. and Dausset, J.: Mixed lymphocyte reactivity of human lymphocytes primed in vitro. I. Secondary response to allogeneic lymphocytes.Eur. J. Immunol. 5:295–301, 1975
Hirschberg, H., Kaakinen, A., and Thorsby, E.: Typing for HLA-D determinants. Comparison of typing results using homozygous stimulating cells and primed cultures.Tissue Antigens 7:213–219, 1975
Mawas, C., Charmot, D., and Sasportes, M.: Secondary response of in vitro-primed human lymphocytes to allogeneic cells. I. Role of HLA antigens and mixed lymphocyte reaction stimulating determinants in secondary in vitro proliferative response.Immunogenetics 2:449–463, 1975
Sheehy, M., Sondel, P., Bach, M., Wank, R., and Bach, F.: HLA-D typing: a rapid assay using primed lymphocytes.Science 188:1308–1310, 1975
Suciu-Foca, N., Susinno, E., Molinaro, A.P., and Rubinstein, P.: Complete typing of the HLA region in families 4. The genetics of HLA-D as seen by the HTC and PLT methods.Transplant. Proc. 9:1751–1754, 1977
Thorsby, E. and Piazza, A.: Joint report from the VI International Workshop Conference. II. Typing for HLA-D (LD-1 or MLC) Determinants.In F. Kissmeyer-Nielsen (ed.):Histocompatibility Testing 1975, pp. 414–458. Munksgaard, Copenhagen, 1976
Wank, R., Schendel, D.J., Hansen, J.A., Yunis, E.J., and Dupont, B.: Two different HLA restimulating determinants separated by recombination and titration.Transplant. Proc. 9:1771–1775, 1977
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Wank, R., Schendel, D.J., Hansen, J.A. et al. The lymphocyte restimulation system: Evaluation by intra-HLA-D group priming. Immunogenetics 6, 107–115 (1978). https://doi.org/10.1007/BF01563901
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DOI: https://doi.org/10.1007/BF01563901