Skip to main content
Log in

Continuous microbial leaching of a pyritic concentrate by Leptospirillum-like bacteria

  • Biotechnology
  • Published:
Applied Microbiology and Biotechnology Aims and scope Submit manuscript

Summary

Continuous leaching of a pyritic flotation concentrate by mixed cultures of acidophilic bacteria was studied in a laboratory scale airlift reactor. Enrichment cultures adapted to the flotation concentrate contained Thiobacillus ferrooxidans and Thiobacillus thiooxidans. During the late stationary growth phase of these thiobacilli growth of Leptospirillum-like bacteria was observed, too. In discontinuous cultivation no significant influence of Leptospirillum-like bacteria on leaching rates could be detected. During continuous leaching at pH 1.5 Leptospirillum-like bacteria displaced Thiobacillus ferrooxidans. The iron leaching rate achieved by Leptospirillum-rich cultures was found to be up to 3.9 times higher than that by Leptospirillum-free cultures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Balashova VV, Vedenina JY, Markosyan GE, Zavarzin GA (1974) The auxotrophic growth of Leptospirillum ferrooxidans. Mikrobiologiya 43:581–585

    Google Scholar 

  • Bosecker K, Neuschütz D, Scheffler U (1978) Microbiological leaching of carbonate-rich german copper shale. In: Murr LE, Torma AE, Brierley JA (eds) Metallurgical applications of bacterial leaching and related microbiological phenomena. Academic Press, New York, pp 389–401

    Google Scholar 

  • Chang YC, Myerson AS (1982) Growth models of the continuous bacterial leaching of iron pyrite by Thiobacillus ferrooxidans. Biotechnol Bioeng 24:889–902

    Google Scholar 

  • Ebner HG (1978) Metal recovery and environmental protection by bacterial leaching of inorganic waste materials. In: Murr LE, Torma AE, Brierley JA (eds) Metallurgical applications of bacterial leaching and related microbiological phenomena. Academic Press, New York, pp 195–206

    Google Scholar 

  • Gormely LS, Duncan DW, Branion RMR, Pinder KL (1975) Continuous culture of Thiobacillus ferrooxidans on a zinc sulfide concentrate. Biotechnol Bioeng 17:31–49

    Google Scholar 

  • Groudev SN (1986) Continuous bacterial leaching of copper sulphide concentrates. In: Lawrence RW, Branion RMR, Ebner HG (eds) Fundamental and applied biohydrometallurgy. Elsevier, Amsterdam, pp 43–50

    Google Scholar 

  • Groudeva VI, Groudev SN (1984) Removal of arsenic from sulphide concentrates by means of microorganisms. In: World Biotech Report USA, Online Publications, pp A57–A65

  • Harrison AP (1986) The phylogeny of iron-oxidizing bacteria. In: Ehrlich HL, Holmes DS (eds) Biotechnology for the mining metal-refining, and fossil fuel processing industries. Biotechnol Bioeng Symp No. 16, John Wiley & Sons, New York, pp 311–318

    Google Scholar 

  • Harrison AP, Norris PR (1985) Leptospirillum ferrooxidans and similar bacteria: some characteristics and genomic diversity. FEMS Microbiol Lett 30:99–102

    Google Scholar 

  • Kandemir H (1983) Fate of sulphide moiety in bacterial oxidation of sulphide minerals: A quantitative approach. In: Rossi G, Torma AE (eds) Recent progress in biohydrometallurgy. Associazione Mineraria Sarda, Iglesias, pp 291–315

    Google Scholar 

  • Kiese S, Ebner HG, Onken U (1980) A simple laboratory airlift fermentor. Biotechnol Lett 2:345–350

    Google Scholar 

  • McElroy RO, Bruynesteyn A (1978) Continuous biological leaching of chalcopyrite concentrates: Demonstration and economic analysis. In: Murr LE, Torma AE, Brierley JA (eds) Metallurgical applications of bacterial leaching and related microbiological phenomena. Academic Press, New York, pp 441–462

    Google Scholar 

  • Myerson AS, Kline PC (1984) Continuous bacterial coal desulfurization employing Thiobacillus ferrooxidans. Biotechnol Bioeng 26:92–99

    Google Scholar 

  • Norris PR (1983) Iron and mineral oxidation with Leptospirillum-like bacteria. In: Rossi G, Torma AE (eds) Recent progress in biohydrometallurgy, Associazione Mineraria Sarda, Iglesias, pp 83–96

    Google Scholar 

  • Norris PR, Kelly DP (1978) Dissolution of pyrite (FeS2) by pure and mixed cultures of some acidophilic bacteria. FEMS Lett 4:143–146

    Google Scholar 

  • Norris PR, Kelly DP (1982) The use of mixed microbial cultures in metal recovery. In: Bull AT, Slater JH (eds) Microbial interactions and communities. Academic Press, London, pp 443–474

    Google Scholar 

  • Oberzill W (1967) Mikrobiologische Analytik. Hans Carl, Nürnberg

    Google Scholar 

  • Sammugasunderam V, Branion RMR, Duncan DW (1985) A growth model for the continuous microbiological leaching of a zinc sulfide concentrate by Thiobacillus ferrooxidans. Biotechnol Bioeng 27:1173–1184

    Google Scholar 

  • Silverman MP, Lundgren DG (1959) Studies on the chemoautotrophic iron bacterium Ferrobacillus ferrooxidans. I. An improved medium and a harvesting procedure for securing high cell yields. J Bacteriol 77:642–647

    Google Scholar 

  • Vian M, Creo D, Dalmastri C, Gionni A, Palazzolo P, Levi G (1986) Thiobacillus ferrooxidans selection in continuous culture. In: Lawrence RW, Branion RMR, Ebner HG (eds) Fundamental and applied biohydrometallurgy. Elsevier, Amsterdam, pp 395–401

    Google Scholar 

  • Vuorinen A, Hiltunen P, Tuovinen OH (1986) Redox and precipitation reactions of iron and uranium solubilized from ore materials. Hydrometallurgy 15:297–301

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Helle, U., Onken, U. Continuous microbial leaching of a pyritic concentrate by Leptospirillum-like bacteria. Appl Microbiol Biotechnol 28, 553–558 (1988). https://doi.org/10.1007/BF00250411

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00250411

Keywords

Navigation