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Optimization of 14C-lysine concentration and specific activity for the radiometric detection of microorganisms

  • Food Microbiology
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European journal of applied microbiology and biotechnology Aims and scope Submit manuscript

Summary

The sensitivity of the radiometric detection of microbial contamination based on the labeling of cells by 14C-lysine was studied as a function of the lysineconcentration and its specific activity for a strain of E. coli and a strain of S. cerevisiae. It was found that best conditions of detection were given by a labelled lysine specific activity of 200 mCi/mmole and a medium radioactivity of 0.2 μCi/ml.

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References

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Authors and Affiliations

  1. Centre de Recherche Agroalimentaire de Quimper, Université de Bretagne Occidentale, F-29191, Quimper, France

    P. Mafart, J. J. Cleret & C. Bourgeois

Authors
  1. P. Mafart
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  2. J. J. Cleret
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  3. C. Bourgeois
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Mafart, P., Cleret, J.J. & Bourgeois, C. Optimization of 14C-lysine concentration and specific activity for the radiometric detection of microorganisms. European J. Appl. Microbiol. Biotechnol. 11, 189–192 (1981). https://doi.org/10.1007/BF00511260

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  • DOI: https://doi.org/10.1007/BF00511260

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