Summary
During batch cultivation of Catharanthus roseus cell suspensions, alkaloids were found in the culture medium after growth had ceased. Resting cell suspensions with high alkaloid content were obtained by transferring the cells to a medium devoid of 2.4 D (2.4 dichlorophenoxy-acetic acid). A production system with continuous feeding was developped to study alkaloid production by these resting cell suspensions.
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Pareilleux, A., Vinas, R. A study on the alkaloid production by resting cell suspensions of Catharanthus roseus in a continuous flow reactor. Appl Microbiol Biotechnol 19, 316–320 (1984). https://doi.org/10.1007/BF00253778
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DOI: https://doi.org/10.1007/BF00253778