Summary
The cells of Lactobacillus casei were adsorbed to Poraver, foam glass particles pretreated with polyethylenimine (PEI). Exposure of cells for a relatively short period to Poraver beads coated with a high concentration of PEI resulted in maximal adsorption with good retention of metabolic activity. The immobilized cells were tested in packed-bed and stirred-tank reactors for lactic acid production. Stirred-tank operations were more effective in terms of productivity but the support was sensitive to attrition. The beads exhibited good mechanical stability to withstand pressure in the packed-bed reactor.
Similar content being viewed by others
References
Boyaval P, Goulet J (1988) Optimal conditions for production of lactic acid from cheese whey permeate by Ca-alginate entrapped Lactobacillus helveticus. Enzyme Microb Technol 10:725–728
D'Souza SF (1989) Immobilized cells: techniques and applications. Indian J Microbiol 29:83–117
D'Souza SF, Kamath N (1988) Cloth bioreactor containing yeast cells immobilized on cotton cloth using polyethylenimine. Appl Microbiol Biotechnol 29:136–140
De Man JC, Rogosa M, Sharpe ME (1960) A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135
Guoqiang D, Kaul R, Mattiasson B (1991) Evaluation of alginate immobilized Lactobacillus casei for lactate production. Appl Microbiol Biotechnol 36:309–314
Krischke W, Schröder M, Trösch W (1991) Continuous production of l-lactic acid from whey permeate by immobilized Lactobacillus casei subsp. casei. Appl Microbiol Biotechnol 34:573–578
Lawrence AJ (1975) Determination of lactic acid in cream. Aust J Dairy Technol 30:14–15
Mattiasson B (ed) (1983) Immobilized cells and cellular organelles, vol 2, CRC Press, Boca Raton, Fla.
Miller GL (1954) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428
Salter GJ, Kell DB, Ash LA, Adams JM, Brown AJ, James R (1990) Hydrodynamic depostion: a novel method of cell immobilization. Enzyme Microb Technol 12:419–430
Van Haecht JL, De Bremaeker M, Rouxhet PG (1984) Immobilization of yeast by adhesion to a support without use of a chemical agent. Enzyme Microb Technol 6:221–227
Van Haecht JL, Bolipombo M, Rouxhet PG (1985) Immobilization of Saccharomyces cerevisiae by adhesion. Treatment of the cells by Al-ions. Biotechnol Bioeng 27:217–224
Author information
Authors and Affiliations
Additional information
Correspondence to: Bo Mattiasson
Rights and permissions
About this article
Cite this article
Guoqiang, D., Kaul, R. & Mattiasson, B. Immobilization of Lactobacillus casei cells to ceramic material pretreated with polyethylenimine. Appl Microbiol Biotechnol 37, 305–310 (1992). https://doi.org/10.1007/BF00210983
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00210983