Abstract
Manganese peroxidases were overproduced byPhanerochaete chrysosporium I-1512 immobilized on nylon net in a bubble-column reactor. This study investigates a new design of bioreactor, a compromise between a pneumatic reactor and an immobilized biofilm reactor. The carrier, a sheet of nylon net, was maintained by a cylindrical stainless-steel frame installed vertically. It was characterized by its hydrophilic nature, its surface morphology and its surface roughness.P. chrysosporium adhesion was highly efficient; mycelial hyphae invaded the tridimensional structure and strengthened the bonding to the network, as shown by electron scanning microscopy. High levels of Mn peroxidases were produced by strain I-1512 under conditions of glycerol and nitrogen sufficiency when the medium was supplemented with phospholipid and veratryl alcohol. Yields of 3600 U/l Mn peroxidase were produced after 95 h of incubation, indicating significant productivity for industrial purposes (900 U day−1 l−1).
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Laugero, C., Sigoillot, J.C., Moukha, S. et al. Selective hyperproduction of manganese peroxidases byPhanerochaete chrysosporium l-1512 immobilized on nylon net in a bubble-column reactor. Appl Microbiol Biotechnol 44, 717–723 (1996). https://doi.org/10.1007/BF00178608
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DOI: https://doi.org/10.1007/BF00178608