Summary
Lysine added to a defined medium at a concentration of 1 mg/ml enhances the capacity of Cephalosporium acremonium to produce cephalosporin C. The lysine effect was accompanied by a delayed differentiation to arthrospores followed by more extensive mycelial fragmentation. Higher lysine concentrations reduced the synthesis of cephalosporin C. The effect of lysine on cephalosporin C synthesis was not strain related and was not elicited by the other amino acids tested. Both lysine stimulation and inhibition were evidenct using resting cells. The lysine inhibition of cephalosporin C synthesis with resting cells was relieved by supplementing the medium with 1 to 2 mg/ml α-aminoadipic acid. This reversal supports the hypothesis that lysine restricts the availability of α-aminoadipic acid for β-lactam biosynthesis.
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Mehta, R.J., Speth, J.L. & Nash, C.H. Lysine stimulation of cephalosporin C synthesis in Cephalosporium acremonium . European J. Appl. Microbiol. Biotechnol. 8, 177–182 (1979). https://doi.org/10.1007/BF00506181
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DOI: https://doi.org/10.1007/BF00506181