Abstract
In this study, erythropoietic activity of bone marrow has been evaluated by ferrokinetics. For that purpose, the data of radioiron disappearance from plasma and its ultimate incorporation in red blood cells after the injection of about 10 μCi of 59Fe tagged autologous plasma, were analysed and fitted to the sum of three exponentials, using a non-linear least square SAAM-25 program on UNIVAC-1108 computer. A function representing time-activity relationship, was constructed. The functional physiological model of iron metabolism, proposed by Cavill and Ricketts, was used to calculate various ferrokinetic parameters in terms of coefficients and exponents of the time-activity function. After identifying the parameters that could be used as indices of erythropoietic activity of bone marrow, the study was repeated in six patients after they had undergone myelostimulative therapy. A distinct correlation was found between ferrokinetic observations and clinical and biochemical findings. It has been demonstrated that in histologically diagnosed cases with bone marrow hypoplasia, a further differentiation between pure aplasia and those with hypoplasia together with ineffective erythropoiesis was possible. This discrimination which is clinically very important, is only possible by means of kinetic investigations.
Similar content being viewed by others
References
Berman, M.: Compartmental analysis in kinetics. In: Computers in biomedical research (R.W. Stacy and B.D. Waxman, eds.), Vol. II, pp. 173–201. New York: Academic Press 1965
Cavill, I., Ricketts, C.: The kinetics of iron metabolism. In: Iron in Biochemistry and Medicine (A. Jacobs and M. Worwood, eds.), pp. 613–647. London: Academic Press 1974
Cook, J.D., Marsaglia, G., Eschbach, J.W., Funk, D.D., Finch, C.A.: Ferrokinetics: A biological model for plasma iron exchange in man. J. Clin. Invest. 49, 197–205 (1970)
Dixon, W.J.: Biochemical computer programs, X-series suppl. Berkeley and Los Angeles: University of California Press 1969
Documenta Geigy, Wissenschaftliche Tabellen, Hrsg. J.R. Geigy AG, 7. Auflage. Stuttgart: Georg Thieme 1975
Finch, C.A., Hosain, F., Morgan, E.H., Marsaglia, G., Giblett, E., Hillman, R.S.: The ferrokinetic approach to anaemia. Series Haemat. 6, 30–40 (1965)
Garby, L., Schneider, W., Sundquist, O., Vuille, J.C.: A ferroerythrokinetic model and its properties. Acta Physiol. Scand. 59 (Suppl. 216), 1 (1963)
Gordon-Smith, E.C., Ahuja, S., Lewis, S.M.: Oxymetholone treatment in aplastic anaemia. Brit. J. Haemat. 20, 670 (1971)
Gupta, M.M., Roth, P., Werner, E., Kaltwasser, J.P.: Changes in iron kinetics by myelostimulative therapy in patients with aplastic anaemia. 15th International Annual Meeting of Society of Nuclear Medicine, Groningen 13–16th Sept. 1977
Najean, Y., Dresch, C., Ardaillou, N., Bernard, J.: Iron metabolism-study of different kinetic models in normal conditions. Am. J. Physiol. 213, 533–546 (1967)
Najean, Y., Dresch, C., Rain, J.D., Failli, A., Glogovski, A.: Erythrokinetic studies of refractory anemias with partial myelo-blastic involvement of the bone marrow. In: Iron metabolism and its disorders (H. Kief, ed.), pp. 203–210. Amsterdam-Oxford: Excerpta Medica 1975
Pollycove, M., Mortimer, R.: The quantitative determination of iron kinetics and hemoglobin synthesis in human subjects. J. Clin. Invest. 40, 753–781 (1961)
Ramsey, W.N.M.: The determination of the total iron binding capacity of serum. Clin. Chim. Acta 2, 221–226 (1957)
Ricketts, C., Jacobs, A., Cavill, I.: Ferrokinetics and erythropoiesis in man: The measurement of effective erythroypoiesis, ineffective erythropoiesis and red cell lifespan using 59Fe. Brit. J. Haemat. 31, 65–75 (1975)
Rodriguez Cuartero, A., Castillo Higueras, P.L., Rodriguez Cuartero, F.: Aplasias medulares: Tratamiento androgenico. Rev. Clin. Españo. 144, 177–180 (1977)
Shahidi, N.T.: Androgens and erythropoiesis. N. Eng. J. Med. 289, 72–80 (1973)
Sharney, L., Gevirtz, N.R., Wasserman, L.R., Schwartz, L., Levitan, R., Mittleman, A., Tendler, D.: Studies in iron kinetics: IV: Calculation of physiological parameters on the basis of multiple-pool models. J. Mt. Sinai Hosp. 32, 338–368 (1965)
Trinder, P.: The improved determination of iron in serum. J. Clin. Path. (Lond.) 9, 170 (1956)
Vincent, P.C., De Gruchy, G.C.: Complications and treatment of aquired aplastic anaemia. J. Haemat. 13, 977 (1967)
Wasserman, L.R., Rashkoff, I.A., Leavitt, D., Mayer, J., Port, S.: The rate of removal of radioactive iron from the plasma-an index of erythropoiesis. J. Clin. Invest. 31, 32–39 (1952)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gupta, M.M., Roth, P., Werner, E. et al. Application of ferrokinetic investigation for differential diagnosis in bone marrow hypoplasia and their clinical relevance. Eur J Nucl Med 4, 17–20 (1979). https://doi.org/10.1007/BF00257564
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00257564