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Enhanced in vivo sensitivity to interferon with in vitro resistant B16 tumor cells in mice

  • Original Articles
  • Melanoma, Antitumor Response, Interferon Resistance, rHulFNαA/D
  • Published:
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Abstract

Mouse B16 melanoma cells rapidly develop resistance to the antiproliferative effects of interferon α (IFNα) and interferon β (IFNβ) when they are exposed to the interferons in vitro. This resistance was characterized to be non-genetic and dose-dependent, and does not alter other IFN-induced effects such as antiviral effects and elevation of 2′,5′-oligoadenylate synthetase activity in IFN-treated cells. The study of these IFN-resistant cells has been extended to an in vivo tumor model. Resistance, if it occurred in vivo, did not adversely affect the survival of IFN-treated mice. Further, IFN-treated mice inoculated with B16 cells that were resistant in vitro (B16αres cells) survived significantly longer than IFN-treated mice inoculated with B16 cells that were sensitive in vitro. The IFN-treated B16αres-inoculated mice had a significantly higher cure rate as well. The prolonged survival of the mice bearing B16αres cell tumors did not seem to be caused by the slower growth rate of the B16αres cells, since experiments performed with a tenfold higher B16αres cell inoculum and a tenfold lower B16 cell inoculum did not show any change in the survival pattern. It is clear that in vitro resistant B16αres cells are more sensitive to antitumor effects induced by IFN in vivo than in vitro sensitive B16 cells.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, The University of Texas Medical Branch, 77555, Galveston, Texas, USA

    Christina M. Fleischmann, G. John Stanton & W. Robert Fleischmann Jr

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  1. Christina M. Fleischmann
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  2. G. John Stanton
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  3. W. Robert Fleischmann Jr
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Additional information

Supported by U. S. Public Health Service grant no. CA50752 awarded by the National Cancer Institute, Department of Health and Human Services (W. R. F.) and by a James W. McLaughlin Fellowship (C. M. F.)

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Fleischmann, C.M., Stanton, G.J. & Fleischmann, W.R. Enhanced in vivo sensitivity to interferon with in vitro resistant B16 tumor cells in mice. Cancer Immunol Immunother 39, 148–154 (1994). https://doi.org/10.1007/BF01533379

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  • DOI: https://doi.org/10.1007/BF01533379

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