Skip to main content
SpringerLink
Log in

Keyhole limpet hemocyanin contains Gal(β1–3)-GalNAc determinants that are cross-reactive with the T antigen

  • Original Article
  • Keyhole Limpet hemocyanin (KLH), T Antigen, Immunotherapy
  • Published:
Cancer Immunology, Immunotherapy Aims and scope Submit manuscript

Abstract

Keyhole limpet hemocyanin (KLH) is widely used as a carrier molecule to enhance immune responses to administered antigens, and for immunotherapy of bladder and renal carcinoma. In the present study we show, using lectin and antibody binding studies, that native KLH contains Gal(β1–3)GalNAc-bearing oligosaccharides, and that immunization with KLH in Lewis rats induces the production of anti-Gal(β1–3)GalNAc antibodies. This might explain the beneficial effect of KLH in bladder cancers that express crossreactive Gal(β1–3)GalNAc determinants or the T antigen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350

    Google Scholar 

  2. Fung PYS, Madej M, Koganty RR, Longenecker BM (1990) Active specific immunotherapy of a murine mammary adenocarcinoma using a synthetic tumor-associated glycoconjugate. Cancer Res 50:4308

    PubMed  Google Scholar 

  3. Grzych J-M, Dissous C, Capron M, Torres S, Lambert P-H, Capron A (1987)Schistosoma mansoni shares a protective carbohydrate epitope with keyhole limpet hemocyanin. J Exp Med 165:865

    PubMed  Google Scholar 

  4. Helling F, Shang A, Calves M, Zhang S, Ren S, Yu RK, Oettgen HF, Livingston PO (1994) GD3 vaccines for melanoma: superior immunogenicity of keyhole limpet hemocyanin conjugate vaccines. Cancer Res 54:197

    PubMed  Google Scholar 

  5. Henningsson CM, Selvaraj S, MacLean GD, Suresh MR, Noujaim AA, Longenecker BM (1987) T cell recognition of a tumor-associated glycoprotein and its synthetic carbohydrate epitopes: stimulation of anticancer T cell immunity in vivo. Cancer Immunol Immunother 25:231

    PubMed  Google Scholar 

  6. Langkilde NC, Wolf H, Clausen H, Ørntoft TF (1992) Human urinary bladder carcinoma glycoconjugates expressing T (Galβ(1–3)GalNAcα1-O-R) and T-like antigens: a comparative study using peanut agglutinin and poly- and monoclonal antibodies. Cancer Res 52:5030

    PubMed  Google Scholar 

  7. Langkilde NC, Wolf H, Clausen H, Kjeldsen T, Ørntoft TF, (1992) Nuclear volume and expression of T-antigen, sialosyl-Tn-antigen, and Tn-antigen in carcinoma of the human bladder. Cancer 69:219

    PubMed  Google Scholar 

  8. Ledeen RW, Yu RK (1982) Gangliosides: structure, isolation and analysis. Methods Enzymol 83:139

    PubMed  Google Scholar 

  9. Longenecker BM, MacLean GD, McEwan AJB, Sykes T, Henningsson C, Suresh MR, Noujaim AA (1988) Synthetic Thomsen Friedenreich antigens for production of anti-carcinoma monoclonal antibodies and generation of anti-carcinoma T cells. UCLA Symp Mol Cell Biol 79:307

    Google Scholar 

  10. MacLean GD, Bowen-Yacyshyn MB, Samuel J, Meikle A, Stuart G, Nation J, Poppema S, Jerry M, Koganty R, Wong T, Longenecker BM (1992) Active immunization of human ovarian cancer patients against a common carcinoma (Thomsen-Friedenreich) determinant using a synthetic carbohydrate antigen. J Immunother 11:292

    PubMed  Google Scholar 

  11. MacLean GD, Reddish M, Koganty RR, Wong T, Gandhi S, Smolenski M, Samuel J, Nabholtz JM, Longenecker BM (1993) Immunization of breast cancer patients using a synthetic sialyl-Tn glycoconjugate plus Detox adjuvant. Cancer Immunol Immunother 36:215

    PubMed  Google Scholar 

  12. Momoi T, Tokunaga T, Nagai Y (1982) Specific interaction of peanut agglutinin with the glycolipid asialo GM1. FEBS Lett 141:6

    PubMed  Google Scholar 

  13. Sadiq SA, Thomas FP, Kilidreas K, Protopsaltis S, Hays AP, Lee KW, Romas SN, Kumar N, Berg L van den, Santoro M, Lange DJ, Younger DS, Lovelace RE, Trojaborg W, Sherman WH, Miller JR, Minuk J, Fehr MA, Roelofs RI, Hollander D, Nichols FT III, Mitsumoto H, Kelley JJ, Swift TR, Munsat TL, Latov N (1990) The spectrum of neurologic disease associated with anti-GM1 antibodies. Neurology 40:1067

    PubMed  Google Scholar 

  14. Sargent ER, Williams RD (1992) Immunotherapeutic alternatives in superficial bladder cancer. Urol Clin N Am 19:581

    Google Scholar 

  15. Singhal A, Fohn M, Hakomori S-H (1991) Induction of α-N-acetylgalactosamine-O-serine/threonine (Tn) antigen-mediated cellular immune response for active immunotherapy in mice. Cancer Res 51:1406

    PubMed  Google Scholar 

  16. Springer GF (1984) T and Tn, general carcinoma autoantigen. Science 224:1198

    PubMed  Google Scholar 

  17. Thomas FP, Trojaborg W, Nagy C, Santoro M, Sadiq SA, Latov N, Hays AP (1991) Experimental autoimmune neuropathy with anti-GM1 antibodies and immunoglobulin deposits at the nodes of Ranvier. Acta Neuropathol 82:378

    PubMed  Google Scholar 

  18. Woodward MP, Young WW Jr, Bloodgood RA (1985) Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation. J Immunol Methods 78:143–153

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Neurology, College of Physicians and Surgeons of Columbia University, Black Bldg Rm 3-323, 650 W. 168th St, 630 West 168th St, 10032, New York, NY, USA

    Itzhak Wirguin, Ljubica Suturkova-Milosević, Chiara Briani & Norman Latov

  2. Department of Neurology, Hebrew University and Hadassah Medical School, Jerusalem, Israel

    Itzhak Wirguin

Authors
  1. Itzhak Wirguin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ljubica Suturkova-Milosević
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chiara Briani
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Norman Latov
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by NIH grant NS11766 and by the William Rosenwald Family Fund Inc.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wirguin, I., Suturkova-Milosević, L., Briani, C. et al. Keyhole limpet hemocyanin contains Gal(β1–3)-GalNAc determinants that are cross-reactive with the T antigen. Cancer Immunol Immunother 40, 307–310 (1995). https://doi.org/10.1007/BF01519630

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01519630

Key words

Search

Navigation