Summary
Experimental arthrosis was induced in previously healthy joints in a group of dogs by injection of fluid aspirated from another joint of the same animal previously damaged by trauma under sterile conditions.
Using electron microscopy progressive degenerative changes were observed at the surface of the joint, in the extracellular matrix, in the chondrocytes and in the pericellular band. The changes increased following serial injections of the joint fluid.
In another series, the joints were injected either with the centrifuged concentrate or the supernatant fluid and a comparison made of the contrasting effects upon the cellular and extracellular structures.
Résumé
Les auteurs ont réalisé une arthrose expérimentale chez le chien en injectant dans une articulation le liquide obtenu par ponction d'une autre articulation du même animal, dont le cartilage a été préalablement scarifié de façon aseptique.
L'examen des surfaces articulaires en microscopie électronique montre des altérations dégénératives progressives au niveau de la matrice extra-cellulaire, des chondrocytes et de la membrane péri-cellulaire. Ces modifications augmentent après des injections répétées de liquide synovial.
Dans une autre série d'animaux, on a injecté soit le culot de centrifugation, soit le surnageant et on a comparé les effets obtenus vis-à-vis des structures cellulaires et extra-cellulaires.
Similar content being viewed by others
References
Dingle JT (1981) Catabolin, a cartilage catabolic factor from synovium. Clin Orthop 15: 219–231
Freeman MAR (1973) Adult articular cartilage. Pitman Medical, Oxford, pp 51–95
Ghadially FN, Roy S (1969) Ultrastructure of synovial joints in health and disease. Butterworths, London, pp. 68–70
Hulth A, Lindberg L, Telhag H (1970) Experimental osteoarthritis in rabbits. Preliminary report. Acta Orthop Scand 41: 522–530
Mankin HJ, Lippiello L (1970) Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips. J Bone Joint Surg 52: 424–434
Reimann J (1973) Experimental osteoarthritis of the knee rabbits induced by alterations of the load bearing. Acta Orthop Scand 44: 496–504
Rothwell AG, Bentley G (1973) Chondrocyte multiplication in osteoarthritic articular cartilage. J Bone Joint Surg 55: 588–594
Salter RJ, Field P (1960) The effects of continuous compression of living articular cartilage. J Bone Joint Surg 42: 31–49
Sledge CB, Blackburn MM (1968) Heparin, Dextran and Dextran Sulfate; effect — of lysosomes from embrionic cartilage. Calcif. Tissue Res 2: 65–68
Sokoloff L (1969) The biology of degenerative joint diseases. The University of Chicago Press, p 28
Zamudio L, Contreras R, Medina FJ, López GR (1976) Producción experimental de — enfermedad articular degenerativa por medios — no mecánicos. Ann Orthop Traumatol 12: 157–166
Zamudio L, Garcí FG, Contreras R, Medina FJ, Smithers ME (1977) tNuevas investigaciones en la producción experimental de — enfermedad articular degenerativa por medios — no mecánicos. Acta Orthop Latino-Americana 4: 234–239
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zamudio, L., Palomo, A.M., García-Felix, G. et al. The ultrastructure of experimental arthrosis in dogs. International Orthopaedics 7, 215–221 (1984). https://doi.org/10.1007/BF00266830
Issue Date:
DOI: https://doi.org/10.1007/BF00266830