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The antigenicity of human hemoglobins

I. The antigenicity of hemoglobin subunit chains demonstrated by urea added immunoelectrophoresis

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Summary

By double diffusion and cellulose acetate membrane immunoelectrophoresis the complexity of the Hb-A, Hb-F antigens and anti-Hb-A antiserum was demonstrated though the anti-Hb-F antiserum contained only 1 antibody against Hb-F. The urea added immunoelectrophoresis which consists of the electrophoretical separation of subunit chains on the cellulose acetate membrane under the existence of 8 m urea and the application of antiserum after the removal of urea was used to investigate the antigenicity of hemoglobin subunit chains. In spite of the complexity of system used, 2 lines, for the fast moving α-chain and for the slow moving β-chain, were demonstrated in anti-Hb-A antiserum and Hb-A system. Also the α-chain line in anti-Hb-A antiserum and Hb-F system and the γ-chain line in anti-Hb-F antiserum and Hb-F system were demonstrated.

Zusammenfassung

Mit der Doppeldiffusion und der Immunoelektrophorese mit der Zellulose-Acetat-Membran wurde die Kompliziertheit der Hb-A, Hb-F-Antigene und des Anti-Hb-A-Antiserums nachgewiesen, obwohl im anti-Hb-F-Antiserum nur ein Antikörper gegen Hb-F enthalten war. Die Antigenität der Hämoglobin-Ketten wurde mit der Harnstoff-Immunoelektrophorese untersucht. Die elektrophoretische Trennung erfolgte auf einer Zellulose-Acetat-Membran unter Einfluß von 8 m Harnstoff und Hinzufügen der Antiseren nach der Entfernung des Harnstoffs. Trotz der Kompliziertheit des benutzten Systems wurden 2 Linien, eine für die schnell wandernde α-Kette-und eine für die langsamere β-Kette, im Anti-Hb-A-Antiserum und Hb-A-System nachgewiesen. Die Linie der α-Kette im Anti-Hb-A-Antiserum und Hb-F-System und die Linie der γ-Kette im Anti-Hb-F-Antiserum und Hb-F-System wurden auch demonstriert.

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Author notes

  1. I. Kimura &  K. Suzuki

    Present address: Department of Serology, Faculty of Medicine, University of Tokyo, Hongo 3-7-1, Bunkyo, Tokyo, Japan

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    1. I. Kimura
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    2. K. Suzuki
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    Kimura, I., Suzuki, K. The antigenicity of human hemoglobins. Blut 19, 129–138 (1969). https://doi.org/10.1007/BF01631127

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    • DOI: https://doi.org/10.1007/BF01631127

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