Summary
In summary only that number of granulocytes can be obtained from normal persons as are contained in an amount of 500 ml fresh blood. But contrary to fresh blood, there is only a very small contamination with lymphocytes and thrombocytes. As known from the literature, the amount of 1.5×109 granulocytes is not sufficient to produce a therapeutic effect. On the other hand, the transfusion of donor granulocytes obtained in this way can only be repeated as often as the transfusion of such amount of erythrocytes is allowed. So the isolation of a sufficient amount of granulocytes from normal subjects for transfusion is not possible with the cell separator by this method.
Zusammenfassung
Es wurde versucht, mit dem IBM-Zellseparator an gesunden Personen für eine Transfusion ausreichend große Mengen an Granulozyten bei optimalem Reinheitsgrad gegenüber den anderen Blutzellen zu isolieren. Es konnten maximal 1,5×109 Granulozyten bei einem Spender isoliert werden, was etwa dem Gehalt einer Frischblutkonserve entspricht. Der Grund für die geringe Ausbeute wird in der Antikoagulierung des Blutes mit ACD gesehen. Bei Verwendung von Heparin als Antikoagulans ist eine wesentliche Verbesserung der Ausbeute zu erwarten.
Literature
Freireich, E. I., R. H. Levin, I. Whang, P. P. Carbone, W. Bronson and E. E. Morse: Ann. N.Y., Acad. Sci.113, 1081–1089 (1963/64).
Morse, E. E., E. I. Freireich, P. P. Carbone, W. Bronson and E. Frei: Transfusion3, 183–192 (1965).
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This investigation was supported by EURATOM and the Society of Radiation Research for Hematology, No. 031-64-IBIAD, as well as by the “Sonderforschungsbereich 37” of the University of Munich.
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Lani, K., Pfisterer, H., Ruppelt, W. et al. Studies on the separation of granulocytes for transfusion from normal persons by the IBM cell separator. Blut 22, 35–38 (1971). https://doi.org/10.1007/BF01633626
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DOI: https://doi.org/10.1007/BF01633626