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Naphthol AS-D chloroacetate esterases in granule extracts from human neutrophil leukocytes

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Summary

Soluble proteins of a granule fraction derived from human neutrophils were subjected to electrophoresis on 15% acrylamide gels at pH 4.3 Several of the protein bands were shown to possess hydrolytic activity on naphthol AS-D chloroacetate at pH 7.4. Electrophoretic movement of NASDCA esterases is similar to those of trypsin, chymotrypsin and elastase.

Zusammenfassung

Lösliche Proteine einer Granulafraktion aus menschlichen Neutrophilen wurden einer Acrylamidgel-Elektrophorese (15% Acrylamidgel bei pH 4,3) unterwerfen. Verschiedene Proteinbanden zeigten eine hydrolytische Aktivität gegenü ber Naphthol-AS-D-Chloracetat bei pH 7,4. Die elektrophoretische Wanderung der Naphthol-AS-D-Chloracetat spaltenden Esterase ist ähnlich der von Trypsin, Chymotrypsin und Elastase.

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Author notes

  1. R. Rindler

    Present address: Department of Internal Medicine, University of Innsbruck, Innsbruck, Austria

Authors and Affiliations

  1. Department of Internal Medicine, University of Innsbruck, Innsbruck, Austria

    F. Schmalzl, Heide Hörtnagl & H. Braunsteiner

  2. Department of Pharmacology, University of Innsbruck, Innsbruck, Austria

    R. Rindler, F. Schmalzl, Heide Hörtnagl & H. Braunsteiner

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  1. R. Rindler
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  2. F. Schmalzl
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  3. Heide Hörtnagl
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  4. H. Braunsteiner
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Rindler, R., Schmalzl, F., Hörtnagl, H. et al. Naphthol AS-D chloroacetate esterases in granule extracts from human neutrophil leukocytes. Blut 23, 223–227 (1971). https://doi.org/10.1007/BF01633774

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  • DOI: https://doi.org/10.1007/BF01633774

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