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Culture of human haemopoietic cells in diffusion chambers

Kultur hämopoetischer Zellen des Menschen in Diffusionskammern

I. Growth of normal peripheral blood cells

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Zusammenfassung

Das Wachstum mononukleärer Zellen des peripheren Blutes in der Diffusionskammerkultur wurde bei 10 Normalpersonen über einen Zeitraum von 17 bis 21 Tagen untersucht. Während der Kultur kam es nach einem initialen Abfall zu einem Anstieg der Gesamtzellzahl mit einem Maximum am 13. Tag. Bei allen untersuchten Individuen zeigte sich eine gute übereinstimmung in den Wachstumsmustern der einzelnen Zellarten. Erhebliche numerische Schwankungen wurden jedoch bei den granulopoetischen Zellen beobachtet, wo sie auf unterschiedliche Stammzellkonzentrationen im Blut der untersuchten Personen zurückgeführt werden müssen. Die Ergebnisse zeigen, da\ die Diffusionskammertechnik eine wertvolle Methode zum Nachweis hämopoetischer Stammzellen und zur Kultur lymphatischer Zellen beim Menschen darstellt.

Summary

Growth of mononuclear cells from human peripheral blood from 10 normal individuals was tested in diffusion chamber culture over a period up to 17 or 21 days. After an initial decrease during the first few days an increase of the total cell number was observed with maximal values on day 13. In all individuals growth of undifferentiated blast cells, lymphocytes, plasma cells, immature and mature granulopoietic cells, macrophages, and megakaryocytes occurred. In all individuals the different cell types had similar growth patterns in diffusion chamber culture. The considerable numerical variations which were seen in the granulopoietic cells were probably due to different stem cell concentrations in the peripheral blood of the investigated individuals. The results indicate that the diffusion chamber technique is a valuable method for the detection of haemopoietic stem cells and the culture of lymphocytic cells in man.

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Authors and Affiliations

  1. Abteilung für Innere Medizin und Hämatologie, Zentrum für Innere Medizin, Kinderheilkunde und Dermatologie der Universität Ulm, Steinhövelstra\e 9, D-7900, Ulm/Donau, Germany

    E. Kurrle, D. Hoelzer & H. Schmücker

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  1. E. Kurrle
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  2. D. Hoelzer
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  3. H. Schmücker
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Supported by the Deutsche Forschungsgemeinschaft (Sonderforschungsbereich 112 „Zell-systemphysiologie”, Projekt B 3)

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Kurrle, E., Hoelzer, D. & Schmücker, H. Culture of human haemopoietic cells in diffusion chambers. Blut 38, 383–389 (1979). https://doi.org/10.1007/BF01007899

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  • DOI: https://doi.org/10.1007/BF01007899

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