Summary
The combination of two-dimensional mini gel electrophoresis with the ‘western blot’ technique proves to be a powerful tool in characterizing lymphoid cells. By testing for kappa and lambda immunoglobulin light chains we were able to differentiate between mono-, oligo-, and polyclonal B-cell diseases. The distribution of the lambda isotypes of 24 cases with chronic lymphocytic B-cell leukemia (B-CLL) tested was not random when compared to the distribution of the lambda light chains in B-lymphocytes of normal persons. This might implicate a genetic link between the lambda loci (chromosome 22) and the development of the lambda-CLL.
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Supported by Deutsche Forschungsgemeinschaft, SFB 31
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Hirsch, F.W., Wagner, M., Bross, K.J. et al. Immunoglobulin light chains in chronic lymphocytic leukemia and related diseases. Blut 49, 339–345 (1984). https://doi.org/10.1007/BF00320208
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DOI: https://doi.org/10.1007/BF00320208