Summary
Bone-marrow biopsies and smears from 59 patients with reactive plas-mocytosis (22), multiple myeloma (24), solitary plasmocytoma (3) and monoclonal gammopathy of undetermined significance (MGUS) (10) were eamined. To demonstrate cytoplasmic immunoglobulin the immunoperoxidase method was applied and evaluated quantitatively. Immunohistology yielded different ranges in κ/λ ratio for reactive plasmocytosis (0.4–3.5), multiple myeloma (≤ 0.1 and ≥ 11.2) and MGUS (0.2–3.0). As a result this method seems to be helpful in characterizing a plasmocytosis and distinguishing overt myeloma from monoclonal gammopathy of undetermined significance and reactive plasmocytosis. A differentiation of monoclonal gammopathy of undetermined significance from reactive plasmocytosis is not possible histologically and immunohistologically.
Similar content being viewed by others
References
Bartl R, Frisch B, Burkhardt R, Fateh-Moghadam A, Mahl G, Gierster P, Sund M and Kettner G (1982) Bone marrow histology in myeloma: its importance in diagnosis, prognosis, classification and staging. Br J Haematol 51: 361–375
Canale DD, Collins RD (1974) Use of bone marrow particle sections in the diagnosis of multiple myeloma. Am J Clin Pathol 61: 382–392
Durie BGM, Salmon SE (1975) A clinical staging system for multiple myeloma. Cancer 36: 842–854
Fadem RS (1952) Differentiation of plasmocytic responses from myelomatous diseases on the basis of bone-marrow findings. Cancer 5: 128–137
Greipp PR, Kyle RA (1983) Clinical, morphological, and cell kinetic differences among multiple myeloma, monoclonal gammopathy of undetermined significance, and smoldering multiple myeloma. Blood 62: 166–171
Hijmans W, Schuit HRE, Hulsing-Hesselink E (1971) An immunofluorescence study on intracellular immunoglobulins in human bone marrow cells. Ann NY Acad Sci 177: 290–305
Hitzman JL, Li CY, Kyle RA (1981) Immunoperoxidase staining of bone marrow sections. Cancer 48: 2438–2446
Hyun BH, Kwa D, Gabaldon H, Ashton JK (1975) Reactive plasmacytic lesions of the bone marrow. Am J Clin Pathol 65: 921–928
Jamshidi K, Swaim WR (1971) Bone marrow biopsy with unaltered architecture — a new biopsy device. J Lab Clin Med 77: 335–342
Kyle RA, Bayrd ED (1976) The monoclonal gammopathies, multiple myeloma and related plasma-cell disorders. Charles C. Thomas, Springfield, pp 116–135
Kyle RA (1982) Monoclonal gammopathy of undetermined significance (MGUS): a review. Clin Haematol 11: 123–150
Lindström PD, Dahlström U (1978) Multiple myeloma or benign monoclonal gammopathy? A study of differential diagnostic criteria in 44 cases. Clin Immunol Immunopathol 10: 168–174
Mepham BL, Frater W, Mitchell BS (1979) The use of proteolytic enzymes to improve immunoglobulin staining by the PAP technique. Histochem J 11: 345–357
Morell A, Skvaril F, Huser HJ, PaiSpengler G, Barandun S (1978) Cytoplasmic immunoglobulins in bone-marrow cells of polyclonal and of monoclonal origin. Acta Haematol 60: 137–147
Pinkus GS, Said JW (1977) Specific identification of intracellular immunoglobulin in paraffin sections of multiple myeloma and macroglobulinemia using an Immunoperoxidase technique. Am J Pathol 87: 47–58
Preud'homme JL, Hurez D, Danon F, Brouet JC, Seligmann M (1976) Intracytoplasmic and surface-bound immunoglobulins in ‘nonsecretory’ and Bence Jones myeloma. Clin Exp Immunol 25: 428–436
Schaefer HE (1983) Histology and Histochemistry in paraffin sections. Verh Dtsch Ges Pathol 67: 6–7
Shimizu K, Ohnishi K, Kunii A (1982) Differentiation of benign monoclonal gammopathy and smouldering multiple myeloma from frank myeloma. Clin Exp Immunol 50: 596–600
Taylor CR, Burns J (1974) The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labeled antibodies. J Clin Pathol 27: 14–20
Taylor CR, Mason DY (1974) The immunohistological detection of intracellular immunoglobulin in formalin-paraffin sections from multiple myeloma and related conditions using the immunoperoxidase technique. Clin Exp Immunol 18: 417–429
Taylor CR, Russell R, Chandor S (1978) An immunohistologic study of multiple myeloma and related conditions, using an immunoperoxidase method. Am J Clin Pathol 70: 612–622
Turesson I (1976) Distribution of immunoglobulin-containing cells in human bone marrow and lymphoid tissues. Acta Med Scand 199: 293–304
Turesson I (1978) Distribution of immunoglobulin-containing cells in bone marrow and lymphoid tissues in patients with monoclonal gammopathy. Acta Med Scand 203: 247–255
Vercelli D, Di Gugliemo R, Guidi G, Scolari L, Buricchi L, Cozzolino F (1980) Bone marrow percentage of plasma cells in the staging of monoclonal gammopathies. Nouv Rev Fr Hematol 22: 139–145
Wintrobe MM (1981) Clinical hematology 8th edn. Lea and Febiger, Philadelphia, pp 1739–1760
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Eckert, F., Schmid, L., Kradolfer, D. et al. Bone-marrow plasmocytosis — An immunohistological study. Blut 53, 11–19 (1986). https://doi.org/10.1007/BF00320578
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00320578