Summary
125I albumin was used to assess the amount of trapped fluid after microhematocrit centrifugation of erythrocytes suspended in buffers of different osmolality. Surprisingly the total amount of trapped fluid per volume unit of packed erythrocytes decreased with decreasing osmolality of the suspending buffer despite erythrocyte swelling. However, if the contribution of the individual erythrocyte to the trapped fluid was calculated, the trapped fluid per erythrocyte did not change between 311 mosm/kg and 256 mosm/kg. For osmolalities below 256 mosm/kg a significant increase of trapped fluid was obtained. It is concluded that the packing ability of erythrocytes is not impaired in suspending fluid of moderate to severe infraphysiological tonicity. The daily clinical experience that considerable degrees of plasma hypoosmolality are tolerated in vivo without hemolysis or impairment of oxygen transport by erythrocytes may be explained by the excellent ability of shape adaptation of erythrocytes to each other and to other surfaces such as vascular endothelia. The method of trapped fluid determination might be of potential value as a complementary method in the evaluation of erythrocyte rheology if the amount of trapped fluid is related to the individual erythrocyte.
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References
Chaplin H, Mollison PL (1952) Correction for plasma trapped in the red cell column of the hematocrit. Blood 7: 1227–1238
Chien S, Dellenback RJ, Usami S, Gregersen MI (1965) Plasma trapping in hematocrit determination. Differences among species. Proc Soc Exp Biol Med 119: 1155–1158
Economou-Mavrou C, Tsenghi C (1965) Plasma trapping in the centrifuged red cells of children with severe thalassemia. J Clin Pathol 18: 203–208
England JM, Walford DM, Waters DAW (1972) Re-assessment of the reliability of the hematocrit. Br J Haematol 23: 247–256
Feo C, Phillips WM (1982) The influence of suspension osmolarity and erythrocytic volume on cell deformability. Nouv Rev Fr Hematol 24: 295–299
Further FW (1956) Effect of spherocytosis on volume of trapped plasma in red cell column of capillary and Wintrobe hematocrits. J Lab Clin Med 48: 421–430
Levinsky NG (1980) Fluid and electrolytes. In: Isselbacher KJ, Adams RD, Braunwald E, Petersdorf RG, Wilson JD (eds) Harrison's Principles of Internal Medicine, 9th Ed. McGraw-Hill, New York, pp 435
Pearson TC, Guthrie DL (1982) Trapped plasma in the microhematocrit. Am J Clin Pathol 78: 770–772
Reinhart WH, Chien S (1985) Roles of cell geometry and cellular viscosity in red cell passage through narrow pores. Am J Physiol 248: C 473–479
Stuart J (1985) Erythrocyte rheology. J Clin Pathol 38: 965–977
Streeten DHP, Moses AM, Miller M (1980) Disorders of the neurohypophysis. In: Isselbacher KJ, Adams RD, Braunwald E, Petersdorf RG, Wilson JD (eds) Harrison's Principles of Internal Medicine, 9th Ed. McGraw-Hill, New York, p 1684
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Stäubli, M., Roessler, B. & Straub, P.W. Fluid trapping of erythrocytes under hypoosmolar conditions. Blut 54, 239–245 (1987). https://doi.org/10.1007/BF00594200
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DOI: https://doi.org/10.1007/BF00594200