Summary
Bone marrow biopsies from 7 patients afflicted with hairy cell leukemia were studied with a panel of monoclonal antibodies at different intervals during alpha recombinant interferon therapy. Monoclonal antibodies Dako-LC and F 8.11.13 were used because they are highly reactive with hairy cells, and 82 H 3 and LeuM 1 were also utilized to identify respectively the residual hemopoietic and myeloid tissue. All antibodies are reactive on sections of formalin-fixed, paraffinembedded biopsy material. Before therapy a uniform hairy cell infiltrate was present and very little tissue was visible even after immunohistochemical staining. During therapy, identification of hairy cells in routine biopsies was difficult, since they were mixed with normal cells and the bone marrow was generally hypoplastic. Immunohistochemical stains seem to allow better identification of hairy cells and a more precise estimate of the degree of repopulation by normal bone marrow cells. It was evident, especially after immunocytochemical analysis, that interferon drastically reduced the extent of the infiltrate and allowed recovery of normal hemopoiesis, but did not produce complete remissions.
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References
Burke JS (1978) The value of bone marrow biopsy in the diagnosis of hairy cell leukemia. Am J Clin Pathol 70: 876–884
Cordell JL, Falini B, Erber WN, Ghosh AK, Abdulaziz Z, MacDonald S, Pulford KAF, Stein H, Mason DY (1984) Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP) complexes. J Histochem Cytochem 32: 219–229
Dalchau R, Kirkley J, Fabre JW (1980) Monoclonal antibody to a human leukocyte specific membrane glycoprotein probably homologous to the leukocyte. Eur J Immunol 10: 737–744
Divine M, Farcet JP, Gourdin MF, Tabilio A, Vasconcelos A, Andre C, Jonault H, Bougnet J, Reyes F (1984) Phenotype study of fresh and cultured hairy cells with the use of immunologic markers and electron microscopy. Blood 64: 547–552
Flandrin G, Signaux F, Castaigne S, Billard C, Aguet M, Boiron M, Falcott E, Degos L (1986) Treatment of hairy cell leukemia with recombinant alpha interferon: I. Quantitative study of bone marrow changes during the first months of treatment. Blood 67: 817–820
Golomb NM, Krant EN, Oviatt DL, Pendegast EJ, Stein RS, Sweet DL (1983) Absence of prolonged benefit of leukopheresis therapy for hairy cell leukemia. Am J Hematol 14: 49–56
Hanjan SN, Kearney JF, Cooper MD (1982) A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. Clin Immunol Immunopathol 23: 172–188
Kurtin PJ, Pinkus GS (1984) Leukocyte common antigen. A diagnostic discriminant between hematopoietic and non hematopoietic neoplasms in paraffin sections using monoclonal antibodies. Hum Pathol 16: 353–365
Posnett DN, Chiorazzi N, Kunkel HG (1982) Monoclonal antibodies with specificity for hairy cell leukemia cells. J Clin Invest 70: 254–261
Parravicini CL, Vago L, Berti E, Cattoretti G, Delia D, Soligo D (1986) Reactivity of common leukocyte panel monoclonal antibodies in normal human lymph node, thymus, spleen, lung, liver, brain, kidney and skin. Immunohistochemical study on paraffine sections with or without enzymatic digestion. Proceedings of the Third International Workshop on Human Leukocyte Differentiation Antigens. Oxford University Press, Oxford, p 37
Quesada JR, Reuben J, Manning JT, Hersh EM, Gutterman JU (1984) Alpha interferon for induction of remission in hairy cell leukemia. N Engl J Med 310: 15–18
Reinherz EL, Haynes BF, Nadler LM, Bernstein ID (eds) (1986) Leukocyte Typing II. Human myeloid and hematopoietic cells. Springer, Berlin Heidelberg New York, p 3
Soligo D, Lambertenghi-Deliliers G, Nava MT, Polli N, Cattoretti G, Polli EE (1986) Scanning immunoelectron microscopy of hairy cell leukemia. Acta Haematol 74: 200–204
Warnke RA, Gatter KC, Falini B, Hildreth P, Woolston RE, Pulford K, Cordell JL, Cohen B, De Wolf-Peters C, Mason DY (1983) Diagnosis of human lymphoma with monoclonal anti-leukocyte antibodies. N Engl J Med 309: 1275–1281
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Supported by the Italian National Research Council CNR. Contract No. 84.00635.44 — Progetto Finalizzato Oncologia and by the “Alberto e Antonietta Matarelli” Foundation
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Soligo, D., Lambertenghi-Deliliers, G., Berti, E. et al. Immunohistochemical evaluation of bone marrow involvement in hairy cell leukemia during interferon therapy. Blut 55, 121–126 (1987). https://doi.org/10.1007/BF00631783
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DOI: https://doi.org/10.1007/BF00631783