Skip to main content
SpringerLink
Log in

Decreased autolysis of dead cells in Adriamycin-resistant lines of the sarcoma 180 of mouse

  • Short Communication
  • Resistance, Autolysis, Drug Trapping
  • Published:
Cancer Chemotherapy and Pharmacology Aims and scope Submit manuscript

Summary

Adriamycin-resistant cell lines of the sarcoma 180 of mouse reveal, besides the known resistance mechanism, a decrease in cellular autolysis compared with the original line. This decrease has been demonstrated by comparative analysis of cell-free supernatants isolated from 7-day-old sensitive and resistant ascites tumors in mice. In order to demonstrate these changes we isolated and quantified the DNA, detecting higher amounts within the supernatants of resistant lines. Furthermore, unspecific DNA-cleaving activity within raw homogenates of the cells is substantially lower in the resistant lines. Residual parts of chromatin may trap the drug and in this way lower its effective concentration. However, the results may also reflect changes in enzymatic complement playing a hitherto unknown role within living cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Danø K (1976) Experimentally developed cellular resistance to daunomycin. Acta Pathol Microbiol Scand [A] Suppl 256

  2. Eksborg SH, Ehrsson H, Ekquist B (1982) Protein binding of anthraquinone glycosides with special reference to Adriamycin. Cancer Chemother Pharmacol 10: 7

    Google Scholar 

  3. Horan PK, Kappler JW (1977) Automated fluorescent analysis for cytotoxicity assays. J Immunol Methods 18: 309

    Google Scholar 

  4. Kunitz M (1950) Crystalline desoxyribonuclease I. Isolation and general properties, spectrophotometric method for the measurements of the desoxyribonuclease activity. J Gen Physiol 33: 349

    Google Scholar 

  5. Meselson MF, Stahl W, Vinograd J (1957) Equlibrium sedimentation of macromolecules in density gradients. Proc Natl Acad Sci (USA) 43: 581

    Google Scholar 

  6. Nelson EM, Tewey KM, Liu LF (1984) Mechanism of antitumor drug action: Poisoning of mammalian DNA topoisomerase II on DNA by 4′-(9-acridinylamino)-methanesulfon-M-anisidide. Proc Natl Acad Sci USA 81: 1361

    Google Scholar 

  7. Noll M (1974) Subunit structure of chromatin. Nature 251: 249

    Google Scholar 

  8. Šonka J, Stoehr M, Vogt-Schaden M, Volm M (1985) Anthracycline resistance and consequences of the in situ — in vitro transfer. Cytometry 6: 437

    Google Scholar 

  9. Volm M, Lindner C (1978) Detection of induced resistance in short-term tests. Adriamycin-resistant sarcoma 180. Z Krebsforsch 91: 1

    Google Scholar 

  10. Zunino F, Gambetta R, DiMarco A, Zaccara A (1972) Interaction of daunomycin and its derivatives with DNA. Biochim Biophys Acta 277: 489

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute for Experimental Pathology, German Cancer Research Center, Im Neuenheimer Feld 280, D-6900, Heidelberg, Federal Republic of Germany

    J. Šonka, A. Günther & M. Volm

Authors
  1. J. Šonka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Günther
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Volm
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Šonka, J., Günther, A. & Volm, M. Decreased autolysis of dead cells in Adriamycin-resistant lines of the sarcoma 180 of mouse. Cancer Chemother. Pharmacol. 18, 83–85 (1986). https://doi.org/10.1007/BF00253071

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00253071

Keywords

Search

Navigation