Skip to main content
SpringerLink
Log in

Transformation of Fulvia fulva, a fungal pathogen of tomato, to hygromycin B resistance

  • Short Communications
  • Published:
Current Genetics Aims and scope Submit manuscript

Summary

A transformation system for the tomato pathogen Fulvia fulva has been developed. Hygromycin B resistant colonies were obtained after treatment of protoplasts with a plasmid containing an E. coli hygromycin B phosphotransferase gene fused to an Aspergillus nidulans promoter. The DNA was stably integrated into the genome. The number and sites of integrations varied among transformants. The demonstration of transformation opens the way for the molecular genetic analysis of the interaction of Fulvia with tomato.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Butler EJ, Jones SG (1949) Plant pathology. MacMillan, London, pp 672–678

    Google Scholar 

  • De Wit PJGM, Spikman G (1982) Physiol Plant Pathol 21:1–11

    Google Scholar 

  • Gritz L, Davies J (1983) Gene 25:179–188

    Google Scholar 

  • Harling R, Kenyon L, Lewis BG, Oliver RP, Turner JG, Coddington A (1987) J Phytopathol (in press)

  • Kaster KR, Burgett SG, Ignolia TD (1984) Curr Genet 8:353–358

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J ((1982) tMolecular cloning. A laboratory manual. lCold Spring Harbor, New York

  • Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, van den Hondel CAMJJ (1987) Gene (in press)

  • Queener SW, Ignolia TD, Skatrud PL, Chapman JL, Kaster KR (1985) A system for genetic transformation of Cephalosporium. Schlessinger D (ed) Microbiology. Amer Soc Micriobiol, pp 468–471

  • Raeder U, Broda P (1985) Lett Appl Microbiol 1:17–20

    Google Scholar 

  • Vollmer SJ, Yanofsky C (1986) Proc Natl Acad Sci USA 83:4869–4873

    Google Scholar 

  • Yoder OC, Weltring K, Turgeon BC, Garber RC, van Etten HD (1986) Technology for molecular cloning of fungal virulence genes. Nato ASI Series, vol HI: Biology and molecular biology of plant-pathogen interactions. Bailey, pp 371–384

Download references

Author information

Authors and Affiliations

  1. Norwich Molecular Plant Pathology Group, School of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich, GB

    R. P. Oliver, I. N. Roberts, R. Harling & L. Kenyon

  2. Medical Biology Laboratory TNO, P.O. Box 45, NL-2280 AA, Rijswijk, The Netherlands

    P. J. Punt, M. A. Dingemanse & C. A. M. J. J. van den Hondel

Authors
  1. R. P. Oliver
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. N. Roberts
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Harling
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. Kenyon
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. P. J. Punt
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. A. Dingemanse
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. C. A. M. J. J. van den Hondel
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oliver, R.P., Roberts, I.N., Harling, R. et al. Transformation of Fulvia fulva, a fungal pathogen of tomato, to hygromycin B resistance. Curr Genet 12, 231–233 (1987). https://doi.org/10.1007/BF00436885

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00436885

Key words

Search

Navigation