Summary
Mutants defective in lysine transport were isolated and characterized. After UV-mutagenesis colonies resistant to thialysine, a toxic analogue of lysine, were isolated and L-lysine uptake into the mutant strains was analyzed. Among the thialysine-resistant strains a group of mutants was found, where the half-saturation constant, KT, of the high-affinity transport system for lysine was higher than in the wild-type, the high-affinity transport system for basic amino acids being specifically affected. This was confirmed by a complementation test in which all the thialysine-resistant strains with a higher KT for lysine uptake belonged to one complementation group. Kinetic and genetic analysis showed that our mutants were identical with can1-1 mutants, showing that a single high-affinity system for the transport of basic amino acids exists in S. pombe.
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Communicated by A. Hinnen
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Sychrová, H., Chevallier, M.R., Horák, J. et al. Thialysine-resistant mutants and uptake of lysine in Schizosaccharomyces pombe . Curr Genet 21, 351–355 (1992). https://doi.org/10.1007/BF00351694
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DOI: https://doi.org/10.1007/BF00351694