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Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2

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Abstract

Plasmids pSP1 and pSP2 are two new Schizosaccharomyces pombe ars1 multicopy vectors with the Saccharomyces cerevisiae LEU2 and URA3 genes as selectable markers. They are derivatives of S. cerevisiae integrative plasmids. These plasmids allow classical molecular genetic techniques, such as mutagenesis, nested deletions and sequencing, to be performed directly.

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References

  • Beach D, Nurse P (1981) Nature 290:140–142

    Google Scholar 

  • Beach D, Durkacz B, Nurse P (1982a) Nature 300:706–709

    Google Scholar 

  • Beach D, Piper M, Nurse P (1982b) Mol Gen Genet 187:326–329

    Google Scholar 

  • Broach JR, Strathern JN, Hicks JB (1979) Gene 8:121–133

    Google Scholar 

  • Gaillardin C, Fournier P, Budar F, Kudla B, Gerbaud C, Heslot H (1983) Curr Genet 7:245–253

    Google Scholar 

  • Henikoff S (1984) Gene 28:351–359

    Google Scholar 

  • Henikoff S (1990) Nucleic Acids Res 18:2961–2966

    Google Scholar 

  • Heyer W-D, Sipiczki M, Kohli J (1986) Mol Cell Biol 6:80–89

    Google Scholar 

  • Hindley J, Phear G, Stein M, Beach D (1987) Mol Cell Biol 7:504–511

    Google Scholar 

  • Losson R, Lacroute F (1983) Cell 32:371–377

    Google Scholar 

  • Matsumoto T, Beach D (1991) Cell 66:347–360

    Google Scholar 

  • Maundrell K, Hutchinson A, Shall S (1988) EMBO J 7:2203–2209

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Sikorski RS, Hieter P (1989) Genetics 122:19–27

    Google Scholar 

  • Weilguny D, Prætorius M, Carr A, Egel R, Nielsen O (1991) Gene 99:47–54

    Google Scholar 

  • Wright A, Maundrell K, Heyer W-D, Beach D, Nurse P (1986) Plasmid 15:156–158

    Google Scholar 

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Communicated by R. Rothstein

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Cottarel, G., Beach, D. & Deuschle, U. Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2. Curr Genet 23, 547–548 (1993). https://doi.org/10.1007/BF00312650

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  • DOI: https://doi.org/10.1007/BF00312650

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