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Sorghum mitochondrial orf25 and a related chimeric configuration of a male-sterile cytoplasm

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Abstract

We describe fundamental characteristics of sorghum mitochondrial orf25,urf209, and a related chimeric configuration,orf265/130, which is restricted to the IS1112C source of cytoplasmic male sterility in sorghum. Transcripts ofurf209 are edited at ten nucleotides, resulting in nine amino-acid changes predicted from genomic sequences. The eDNA-predicted polypeptide product is 23.6 kDa, while Western blot analyses identify a product of 20 kDa. Transcription ofurf209 is characterized by one or two transcripts, dependent on nuclear background, but this difference is not related to male fertility status. Theorf265/130 chimeric region includes 288 by 95% identical to sequences 5′ to maize T-cytoplasmT-urf13 andatp6, which includes a common transcription initiation site, and terminates with a recombinational event involvingurf209. Theurf209 similarity extends 189 bp, followed by sequences duplicated 5′ to sorghumatp6-2. Sequences immediately 3′ to theatp6-2 similarity include a second inframe start codon, definingorf130. Structural features 5′ toorf130 are shared with motifs found 5′ to several translated mitochondrial open reading frames. Theorf265/orf130 configuration is uniquely transcribed, and transcripts oforf130 exhibit one silent RNA editing event. Transcription in somatic cells is not altered by male fertility status.

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Authors and Affiliations

  1. Department of Plant Pathology and Plant Molecular and Cellular Biology Program, University of Florida, 32611, Gainesville, FL, USA

    Hoang Van Tang, Figuhr R. Muza & Bin Yan

  2. USDA-ARS, Crop Genetic and Environmental Research Unit, University of Florida, 32611, Gainesville, FL, USA

    Daryl R. Pring

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  1. Hoang Van Tang
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  2. Daryl R. Pring
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  3. Figuhr R. Muza
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  4. Bin Yan
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Communicated by K. J. Newton

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Van Tang, H., Pring, D.R., Muza, F.R. et al. Sorghum mitochondrial orf25 and a related chimeric configuration of a male-sterile cytoplasm. Curr Genet 29, 265–274 (1996). https://doi.org/10.1007/BF02221557

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  • DOI: https://doi.org/10.1007/BF02221557

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