Abstract
Glutamine uptake in S. cerevisiae is mediated by at least three transporters: high- and low-affinity glutamine permeases and the general amino-acid permease. We have isolated the gene encoding the high-affinity glutamine permease and named it GNP1. The amino-acid sequence of GNP1, and its hydropathy profile of 12 transmembrane domains, closely resemble those of known amino-acid permeases. The Km of GNP1 for glutamine uptake was determined to be 0.59 mM. Cells lacking GNP1 exhibit reduced levels of glutamine transport, and are resistant to a toxic analog of glutamine, L-glutamic acid γ-monohydroxamate. Unlike other amino-acid permeases, whose expression is nitrogen-source limited, GNP1 is expressed on both rich and poor nitrogen sources.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 27 February / 30 March 1996
Rights and permissions
About this article
Cite this article
Zhu, X., Garrett, J., Schreve, J. et al. GNP1, the high-affinity glutamine permease of S. cerevisiae. Curr Genet 30, 107–114 (1996). https://doi.org/10.1007/s002940050108
Issue Date:
DOI: https://doi.org/10.1007/s002940050108