Abstract
The sD gene of Aspergillus nidulans has been cloned by heterologous screening of rationally selected cosmids. Co-transformation of the sD50 mutant JMP1 confirmed the presence of a functional gene. Sequence analysis determined this gene to be 680 bp in length, containing a 59-bp intron and encoding a protein of 206 amino acids. A protein-sequence comparison revealed a similarity to the C-terminal region of ATP sulphurylase, the sC gene product. Further sequence comparison revealed differences in a consensus sequence ATP-binding motif, indicating non-functionality of the APS kinase-like domain of ATP sulphurylase, and confirms sD as the gene encoding APS kinase in A. nidulans.
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Received: 17 April / 29 August 1997
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Clarke, D., Newbert, R. & Turner, G. Cloning and characterisation of the adenosyl phosphosulphate kinase gene from Aspergillus nidulans. Curr Genet 32, 408–412 (1997). https://doi.org/10.1007/s002940050295
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DOI: https://doi.org/10.1007/s002940050295