Skip to main content
SpringerLink
Log in

Localization of genes for coupling factor subunits on the spinach plastid chromosome

  • Published:
Current Genetics Aims and scope Submit manuscript

Summary

  1. 1)

    Messenger RNA obtained from spinach cotyledons directs the synthesis of all five CF1 subunits in vitro in a rabbit reticulocyte translation system. The alpha, beta and epsilon subunit polypeptides were found as translation products from ptRNA and whole-cell poly A−-RNA. The gamma and delta subunits were synthesized from whole-cell poly A+-RNA as precursors of substantially greater molecular weight indicating that they originate in the nucleus and are imported into the chloroplast. High resolution electrophoresis, immunoprecipitation with antibodies against individual CF1 subunits (Nelson et al. 1980), and proteolytic peptide mapping were employed to identify the products.

  2. 2)

    The genes for alpha, beta and epsilon subunits of CF1 were located by hybrid-selected translation with matrix-immobilized ptDNA fragments of known map position. The genes for all three CF1 subunit polypeptides are located in the large single-copy segment (cf. Herrmann et al. 1980b) of the circular ptDNA and each gene appears to be present once on the chromosome. The genes for the beta and epsilon subunits lie near each other in immediate vicinity to the structural gene for the large subunit of ribulose bisphosphate carboxylase/oxygenase. The gene for the alpha subunit is separated by approximately 40 kbp from this gene cluster, and located near the gene for the 32 kd photosystem II polypeptide (Driesel et al. 1980).

  3. 3)

    Restriction fragments of spinach ptDNA with CF1 subunit genes were cloned into pBR 322 and used to construct detailed maps.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ptDNA:

plastid, chloroplast DNA

ptRNA:

plastid, chloroplast RNA

CF1 :

Coupling Factor, extrinsic part of the chloroplast ATP synthetase complex

RuBPcase/oase:

ribulose-1,5-bisphosphate carboxylase/oxygenase

LSU:

large subunit of RuBPcase/oase

SSU:

small subunit of RuBPcase/oase

IgG:

immunoglobulin

Md:

mega dalton

bp:

base pairs

kbp:

kilobase pairs

EDTA:

ethylenediamine tetraacetate, disodium salt

SDS:

sodium dodecylsulfate

PIPES:

piperazine-N,N′-bis(2-ethanesulfonic acid)

MES:

2-(N-morpholino)ethanesulfonic acid

Tricine:

N-tris(hydroxymethyl) methylglycine

SSC:

0.15 NaCl, 0.015 M tri-sodium citrate

References

  • Alwine JC, Kemp DJ, Stark GR (1977) Proc Natl Acad Sci USA 74:5350–5354

    Google Scholar 

  • Anderson JM (1981) FEBS Lett 124:1–10

    Google Scholar 

  • Bedbrook JR, Link G, Coen DM, Bogorad L, Rich A (1978) Proc Natl Acad Sci USA 75:3060–3064

    Google Scholar 

  • Binder A, Jagendorf A, Ngo E (1978) J Biol Chem 253:3094–3100

    Google Scholar 

  • Bohnert HJ, Driesel AJ, Herrmann RG (1976) In: Bücher Th, Neupert W, Sebald W, Werner S (eds) Genetics and Biogenesis of Chloroplasts and Mitochondria. Elsevier/North Holland Press, Amsterdam, pp 629–636

    Google Scholar 

  • Bottomley W, Whitfeld PR (1979) Eur J Biochem 93:31–39

    Google Scholar 

  • Bywater R (1978) In: Epton R (ed) Chromatography of Synthetic and Biological Polymers. Ellis Horwood, Chichester UK, pp 337–346

    Google Scholar 

  • Chamberlain JP (1979) Anal Biochem 98:132–135

    Google Scholar 

  • Chua NH, Schmidt GW (1978) Proc Natl Acad Sci USA 75:6110–6114

    Google Scholar 

  • Chua NH, Blomberg F (1979) J Biol Chem 254:215–223

    Google Scholar 

  • Chua NH, Schmidt GW (1979) J Cell Biol 81:461–483

    Google Scholar 

  • Chua NH (1980) In: Colowick S, Kaplan N (eds) Methods in Enzymology. Photosynthesis and Nitrogen Fixation, Vol 69, Part C. Academic Press, New York London Toronto Sydney San Francisco, pp 434–446

    Google Scholar 

  • Cleveland DW, Fischer SW, Kirschner MW, Laemmli UK (1977) J Biol Chem 252:1102–1106

    Google Scholar 

  • Covey SN, Grierson D (1976) Eur J Biochem 63:599–606

    Google Scholar 

  • Crouse EJ, Schmitt JM, Bohnert HJ, Gordon K, Driesel AJ, Herrmann RG (1978) In: Akoyunoglou G, Argyroudi-Akoyunoglou JH (eds) Chloroplast Development. Elsevier/North Holland Press, Amsterdam New York Oxford, pp 565–572

    Google Scholar 

  • Doherty A, Gray JC (1980) Eur J Biochem 108:131–136

    Google Scholar 

  • Driesel AJ (1979) Thesis, University of Düsseldorf

  • Driesel AJ, Crouse EJ, Gordon K, Bohnert HJ, Herrmann RG, Steinmetz A, Mubumbila M, Keller M, Burkhard G, Weil JH (1979) Gene 6:285–306

    Google Scholar 

  • Driesel AJ, Speirs J, Bohnert HJ (1980) Biochim Biophys Acta 610:297–310

    Google Scholar 

  • Ellis RJ (1977) Biochim Biophys Acta 463:185–215

    Google Scholar 

  • Harboe N, Inglid A (1973) Scand J Immunol 2, Suppl 1:161–164

    Google Scholar 

  • Harms E, Rohde W, Bosch F, Scholtissek C (1978) Virology 86:413–422

    Google Scholar 

  • Heber U (1973) Biochim Biophys Acta 305:140–152

    Google Scholar 

  • Herrmann RG (1981) In: Edelman M, Hallick R Chua NH (eds) Methods in Chloroplast Molecular Biology. Elsevier/North Holland Press, Amsterdam (in press)

    Google Scholar 

  • Herrmann RG, Whitfeld PR (1981) In: Edelman M, Hallick R, Chua NH (eds) Methods in Chloroplast Molecular Biology. Elsevier/North Holland Press, Amsterdam (in press)

    Google Scholar 

  • Herrmann RG, Seyer P, Schedel R, Gordon K, Bisanz C, Winter P, Hildebrandt JW, Wlaschek M, Alt J, Driesel AJ, Sears BB (1980a) In: Bücher Th, Sebald W, Weiss H (eds) Biological Chemistry of Organelle Formation. Springer Verlag, Berlin Heidelberg New York, pp 97–112

    Google Scholar 

  • Herrmann RG, Whitfeld PR, Bottomley W (1980b) Gene 8:179–191

    Google Scholar 

  • Herrmann RG, Possingham JV (1980) In: Reinert J (ed) Results and Problems in Cell Differentiation, Vol 10. Springer Verlag, Berlin Heidelberg New York, pp 45–96

    Google Scholar 

  • Katz L, Kingsbury DT, Helinski DR (1973) J Bacteriol 114:557–591

    Google Scholar 

  • Kessler SW (1975) J Immunol 115:1617–1624

    Google Scholar 

  • Link G, Bogorad L (1980) Proc Natl Acad Sci USA 77:1832–1836

    Google Scholar 

  • Mattoo AK, Pick U, Hoffmann-Falk H, Edelman M (1981) Proc Natl Acad Sci USA 78:1572–1576

    Google Scholar 

  • Miller KR, Staehelin LA (1976) J Cell Biol 68:30–42

    Google Scholar 

  • Mendiola-Morgenthaler LR, Morgenthaler JJ, Price CA (1976) FEBS Lett 62:96–99

    Google Scholar 

  • Nechushtai R, Nelson N, Mattoo AK, Edelman M (1981) FEBS Lett 125:115–119

    Google Scholar 

  • Nelson N (1981) In: Sanadi DR (ed) Current Topics in Bioenergetics, Vol 11. Academic Press, New York London Toronto Sydney San Francisco, pp 1–33

    Google Scholar 

  • Nelson N, Hauska G (1979) In: Lee CP, Schatz G, Ernster L (eds) Membrane Bioenergetics. Addison-Wesley Publishing Company, London Amsterdam Don Mills Sydney Tokyo, pp 189–202

    Google Scholar 

  • Nelson N, Schatz G (1979) Proc Natl Acad Sci USA 76:4365–4369

    Google Scholar 

  • Nelson N, Nelson H, Schatz G (1980) Proc Natl Acad Sci USA 77:1361–1364

    Google Scholar 

  • Noyes BE, Stark GR (1975) Cell 5:301–310

    Google Scholar 

  • Pelham HRB, Jackson RJ (1976) Eur J Biochem 67:247–256

    Google Scholar 

  • Pick U, Racker E (1979) J Biol Chem 254:2793–2799

    Google Scholar 

  • Ricciardi RP, Miller JS, Roberts BE (1979) Proc Natl Acad Sci USA 76:4927–4931

    Google Scholar 

  • Seyer P, Kowallik KV, Herrmann RG (1981) Current Genetics 3:189–204

    Google Scholar 

  • Stafford DW, Bieber D (1975) Biochim Biophys Acta 378:18–21

    Google Scholar 

  • Strotmann H, Hesse H, Edelmann K (1973) Biochim Biophys Acta 314:202–210

    Google Scholar 

  • Towbin H, Staehelin T, Gordon J (1979) Proc Natl Acad Sci USA 76:4350–4354

    Google Scholar 

  • Trebst A (1974) Ann Rev Plant Physiol 25:423–458

    Google Scholar 

  • Westhoff P, Zetsche K (1981) Eur J Biochem 116:261–267

    Google Scholar 

  • Williams JG, Lloyd MM, Devine JM (1979) Cell 17:903–913

    Google Scholar 

  • Whitfeld PR, Bottomley W (1980) Biochemistry International 1:172–178

    Google Scholar 

  • Woolford JL, Rosbash M (1979) Nucl Acids Res 6:2483–2497

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Botanisches Institut der Universität, Universitätsstr. 1, 4 Düsseldorf 1, Germany

    P. Westhoff & R. G. Herrmann

  2. Department of Biology, Technion, Haifa, Israel

    N. Nelson

  3. Institut für Genetik der Universität, Universitätsstr. 1, 4 Düsseldorf 1, Germany

    H. Bünemann

Authors
  1. P. Westhoff
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Nelson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Bünemann
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. G. Herrmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Westhoff, P., Nelson, N., Bünemann, H. et al. Localization of genes for coupling factor subunits on the spinach plastid chromosome. Current Genetics 4, 109–120 (1981). https://doi.org/10.1007/BF00365689

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00365689

Key words

Search

Navigation