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HLA class II genes and antibodies against recombinant U1-nRNP proteins in patients with systemic lupus erythematosus

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Abstract

To investigate a possible involvement of HLA-class II alleles in the genetic predisposition for the formation of anti-U1-nRNP antibody in systemic lupus erythematosus (SLE), genomic DNA of 178 patients was typed for the DRB1, DQA1 and DQB1 alleles using a polymerase chain reaction (PCR) and non-radioactive-oligonucleotide typing. Antibodies against recombinant U1-nRNP proteins (U1-A- U1-C-and 70K-protein) were determined by ELISA. Anti-U1-C antibody was found in 26 (14.7%), anti-U1-A in 34 (19.2%) and anti-70K in 17 (9.6%) patients. A joint occurrence was observed for these antibodies against the recombinant U1-nRNP proteins: anti-U1-C and anti-U1-A antibodies occurred together more frequently than alone and than together with anti-U1-70K antibodies. The frequency of DRB1 * 04 was slightly increased in the patients with anti-U1-C as compared to the patients without anti-U1-C (P<0.05, Pcorr=n.s., RR=2.4). The DQA1 * 0301 allele, which is in linkage disequilibrium with DRB1 * 04, is found more frequently in anti-U1-C-positive than in antibody-negative patients. The DQB1 * 0303 allele, detected in 12 of 176 SLE patients, was absent in the patients with any of the antibodies against the U1-nRNP proteins. All these deviations may be due to chance alone. We concluded that the presence of antibodies against recombinant U1-nRNP proteins was not significantly associated with any HLA DRB1, DQA1 and DQB1 allele in our group of SLE patients.

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Authors and Affiliations

  1. Labor für Immungenetik, Kinderpoliklinik der Universität München, Pettenkoferstrasse 8a, D-80336, München, Germany

    Z. Yao, E. Keller, C. Nevinny-Stickel, E. D. Albert, M. Baur, A. Corvetta, J. Frey, J. R. Kalden, F. Krapf, B. Lang, G. G. de Lange, H. J. Lakomek, H. H. Peter, K. Pirner, C. Rittner, E. Röther, P. Schneider, S. Seuchter, W. Stangel, C. Specker & P. Späth

  2. Privates Institut für Immunologie und Molekulargenetik GmbH, Karlsruhe, Germany

    H. P. Seelig, H. Ehrfeld & M. Renz

  3. Zentrallabor, Zentralkrankenhaus Reinkenheide, Bremerhaven, Germany

    K. Hartung & H. Deicher

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  1. Z. Yao
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  2. H. P. Seelig
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  4. M. Renz
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  5. K. Hartung
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  6. H. Deicher
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  7. E. Keller
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  8. C. Nevinny-Stickel
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  9. E. D. Albert
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  10. M. Baur
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  15. B. Lang
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  16. G. G. de Lange
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Yao, Z., Seelig, H.P., Ehrfeld, H. et al. HLA class II genes and antibodies against recombinant U1-nRNP proteins in patients with systemic lupus erythematosus. Rheumatol Int 14, 63–69 (1994). https://doi.org/10.1007/BF00300249

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  • DOI: https://doi.org/10.1007/BF00300249

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