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Detection of anti-bovine β2-glycoprotein I antibody in sera from patients with antiphospholipid syndrome

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Abstract

We studied and characterized anti-bovine β2 I antibodies (aBβ2-GPI) in sera from patients with antiphospholipid syndrome (APS) by ELISA. Bovine β2-glycoprotein I β2-GPI was purified by heparin affinity and DEAE ion-exchange chromatography, and identified on immunoblots using a monoclonal antibody against human β2-GPI and by amino acid sequence analysis. aBβ2-GPI levels in the sera from 36 APS patients were measured by ELISA using purified bovine β2-GPI as an antigen. The mean±standard deviation level of aBβ2-GPI was 17.4±22.0 units in the 58% of APS patients who were positive. There was a significant correlation (P=0.003) between aBβ2-GPI and anticardiolipin antibody (aCL) levels. aBβ2-GPI from the sera of patients with APS was inhibited by bovine β2-GPI itself. Purified IgG from the sera of patients with APS showed that bovine β2-GPI was capable of acting as a cofactor for aCL. Purified bovine β2-GPI was useful antigen for conventional ELISA. aBβ2-GPI may contribute to the further development of aCL analysis and to the understanding of the pathogenesis of APS.

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Authors and Affiliations

  1. Division of Rheumatology, Department of Internal Medicine, Kitasato University School of Medicine, 1-15-1, Kitasato, 228, Sagamihara, Kanagawa, Japan

    M. Nomura, J. Okada & H. Kondo

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  1. M. Nomura
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  2. J. Okada
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  3. H. Kondo
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Nomura, M., Okada, J. & Kondo, H. Detection of anti-bovine β2-glycoprotein I antibody in sera from patients with antiphospholipid syndrome. Rheumatol Int 15, 1–8 (1995). https://doi.org/10.1007/BF00286762

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  • DOI: https://doi.org/10.1007/BF00286762

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