Skip to main content
SpringerLink
Log in

In vitro morphogenetic response and distribution of endogenous plant hormones in hypocotyl segments of snapdragon (Antirrhinum majus L.)

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Snapdragon seedlings, 20 mm in length, were cut into 5 segments from the cotyledon to the root, which were cultured in vitro on hormone-free MS medium. Adventitious shoot formation was highest in the basal hypocotyl segments with stimulation by the addition of BA. Endogenous cytokinins were higher in the basal hypocotyl segments than in the two upper hypocotyl segments, whereas auxin content was higher in the two upper than in the basal hypocotyl segments. Ratios of cytokinins to auxin were also the highest in the basal hypocotyl segment. A general principle in in vitro culture that a high concentration of cytokinin and a low concentration of auxin promotes the induction of shoot morphogenesis was confirmed from measurements of endogenous growth regulator concentrations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ammirato PV (1986) In: Withers LA, Alderson PG (eds) Plant tissue culture and its agricultural application. Butterworth, London, pp 23–45

    Google Scholar 

  • Arrillaga I, Brisa MC, Segura J (1986) J Plant Physiol 124:425–430

    Google Scholar 

  • Atkinson NJ, Ford-Lloyd BV, Newburry HJ (1989) Plant Cell Tissue and Organ Culture 17:59–70

    Google Scholar 

  • Campbell RA, Durzan DJ (1975) Can J Bot 53:1652–1657

    Google Scholar 

  • Fahn A (1982) Plant Anatomy, 3rd edn. Pergamon Press, Oxford

    Google Scholar 

  • Fári M, Czakó M (1981) Scientia Hortic 15:207–213

    Google Scholar 

  • Gamborg OL, Shyluk JP (1976) Bot Gaz 137:301–306

    Google Scholar 

  • Gunay AL, Rao PS (1978) Plant Sci Lett 11:365–372

    Google Scholar 

  • Horemans S, Van Onckelen HA, De Greef JA (1986) J Exp Bot 37:1525–1532

    Google Scholar 

  • Iino M, Carr DJ (1982) Plant Physiol 69:950–956

    Google Scholar 

  • Kamat MG, Rao PS (1978) Plant Sci Lett 13:57–65

    Google Scholar 

  • Kameya T, Widholm J (1981) Plant Sci Lett 21:289–294

    Google Scholar 

  • Kohlenbach HW (1977) In: Barz W, Reinhard E, Zenk MH (eds) Plant tissue culture and its biotechnical application. Springer-Verlag, Berlin, pp 355–366

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Pfister JM, Widholm JM (1984) HortScience 19:852–854

    Google Scholar 

Download references

Author information

Author notes

  1. Koichi Wada

    Present address: Nihon Nohyaku Co., Ltd., Kawachinagano 586, Osaka, Japan

  2. Shunpei Uemoto

    Present address: Oita Junior College, Oita 870, Japan

Authors and Affiliations

  1. Laboratory of Horticultural Science, Faculty of Agriculture, Kyushu University, 812, Fukuoka, Japan

    Hiroshi Okubo, Koichi Wada & Shunpei Uemoto

Authors
  1. Hiroshi Okubo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Koichi Wada
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shunpei Uemoto
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by J. R. Widholm

Rights and permissions

Reprints and permissions

About this article

Cite this article

Okubo, H., Wada, K. & Uemoto, S. In vitro morphogenetic response and distribution of endogenous plant hormones in hypocotyl segments of snapdragon (Antirrhinum majus L.). Plant Cell Reports 10, 501–504 (1991). https://doi.org/10.1007/BF00234582

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00234582

Keywords

Search

Navigation