Abstract
Thalictrum minus cells immobilized in calcium-alginate beads were cultured in a newly devised bioreactor for the purpose of producing berberine. This system could prevent cells from leaking out of the beads, allowing them to release most of the berberine synthesized into the liquid medium. Furthermore, the biosynthetic capability of the immobilized cells was found to be comparable to that of freely suspended cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Brodelius P (1985) Trends in Biotechnology 3: 280–285
Kierstan M, Bucke C (1977) Biotech. Bioeng. 19: 387–397
Nakagawa K, Konagai A, Fukui H, Tabata M (1984) Plant Cell Reports 3: 254–257
Nakagawa K, Fukui H, Tabata M (1986) Plant Cell Reports 5: 69–71
Author information
Authors and Affiliations
Additional information
Communicated by F. Constabel
Rights and permissions
About this article
Cite this article
Kobayashi, Y., Fukui, H. & Tabata, M. An immobilized cell culture system for berberine production by Thalictrum minus cells. Plant Cell Reports 6, 185–186 (1987). https://doi.org/10.1007/BF00268474
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00268474