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Characterization of soybean tissue culture cell lines resistant to methionine analogs

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Abstract

Several hundred soybean [Glycine max (L.) Merr.] cell lines resistant to ethionine were isolated either with or without chemical mutagenesis. of these, 26 were found to contain 2 to 22 times higher than normal levels of uncombined methionine. These 26 cell lines also contained higher than normal levels of S-adenosylmethionine and S-methylmethionine, but the levels of free lysine, threonine, cysteine, valine, tyrosine and phenylalanine were not elevated. Isoleucine levels were only slightly elevated. These results suggest that the regulation of methionine synthesis in vivo is more likely to be later in the pathway (after homoserine phosphate) than early in the pathway.

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Abbreviations

AdoMet:

S-adenosylmethionine

SMM:

S-methylmethionine

trp:

tryptophan

met:

methionine

thr:

threonine

val:

valine

phe:

phenylalanine

lys:

lysine

ile:

isoleucine

cys:

cysteine

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Authors and Affiliations

  1. Agricultural Research Service, United States Department of Agriculture, U.S. Plant, Soil & Nutrition Laboratory, Tower Road, 14853, Ithaca, NY, USA

    James T. Madison & John F. Thompson

Authors
  1. James T. Madison
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  2. John F. Thompson
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Additional information

Communicated by E. D. Earle

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Madison, J.T., Thompson, J.F. Characterization of soybean tissue culture cell lines resistant to methionine analogs. Plant Cell Reports 7, 473–476 (1988). https://doi.org/10.1007/BF00272734

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  • DOI: https://doi.org/10.1007/BF00272734

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