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Larval salt gland ofArtemia salina nauplii

Regulation of protein synthesis by environmental salinity

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Summary

Naupliar brine shrimp (Artemia salina) have been used to study the salt-dependent regulation of protein synthesis. Measurement of thein vivo rates of protein synthesis was found to be very complex and dependent upon the leucine concentration of the external medium, rate of leucine entry and time of equilibration between various internal pools of leucine. Techniques were developed which permitted the measurement of rates of incorporation ofl4C-L-leucine into naupliar protein at various salinities under conditions that provided the organisms with a constant internal specific activity. It was found that salinities over 0.25 M NaC1 caused decreased rates of protein biosynthesis. A comparison of the rate of protein synthesis in the presence of chloramphenicol, cycloheximide and puromycin indicated that qualitative as well as quantitative changes in synthesis of proteins was directed by the external salinity. A feed-back mechanism based on the partitioning of available energy (ATP) between ion transport and protein synthesis is hypothesized.

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Authors and Affiliations

  1. Department of Zoology, Oregon State University, Corvallis, Oregon

    F. P. Conte, G. L. Peterson & R. D. Ewing

Authors
  1. F. P. Conte
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  2. G. L. Peterson
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  3. R. D. Ewing
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Additional information

This work was supported by AEC grant RLO 2227-T13-1. The authors wish to express their gratitude to Prof. Robert R. Beoker and Robert L. Howard, Department of Biochemistry and Biophysics, for the technical assistance and use of the automatic amino acid analyzer in collecting data presented in Table 1 and Table 2.

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Conte, F.P., Peterson, G.L. & Ewing, R.D. Larval salt gland ofArtemia salina nauplii. J. Comp. Physiol. 82, 277–289 (1973). https://doi.org/10.1007/BF00694240

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  • DOI: https://doi.org/10.1007/BF00694240

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