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The effects of octopamine on juvenile hormone biosynthesis, electrophysiology, and cAMP content of the corpora allata of the cockroach Diploptera punctata

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Summary

Juvenile hormone production by the corpora allata of the adult female cockroach, Diploptera punctata, can be modulated by treatment with the biogenic amine, octopamine. Endogenous octopamine has been identified within the CA, using HPLC and electrochemical detection. Treatment with octopamine results in a sinusoidal, dose-dependent inhibition of JH biosynthesis by CA from day 2 virgin females, with maximal inhibition occurring at 10-10 M and 10-4 M. In day 4 and day 8 mated female corpora allata octopamine inhibited JH biosynthesis at 5·10-5 M. Although the elevation of either cAMP or cGMP within the CA is known to be associated with an inhibition of JH biosynthesis, treatment with high concentrations of octopamine results in an increase in the level of cAMP but not cGMP. This effect is both dose- and time-dependent.

Octopamine treatment also initiates changes in the passive membrane responses of the CA. Superfusion of CA with octopamine results in a pronounced hyperpolarization of CA cells and an increase in the electrotonic potential (indicative of the degree of electrical coupling between CA cells). This effect could be blocked by the octopamine receptor blocker phentolamine. Treatment with octopamine or phentolamine also blocked the hyperpolarization of CA cells normally associated with electrical stimulation of the axon tracts innervating the CA.

We hypothesize that octopamine may be a natural neuromodulator of JH production by CA, regulating ion channels in CA cells themselves as well as release of the inhibitory neuropeptide, allatostatin, from the terminals within the CA.

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Abbreviations

4-AP :

4-aminopyridine

CA :

corpora allata

CC :

corpora cardiaca

cAMP :

cyclic adenosine monophosphate

cGMP :

cyclic guanosine monophosphate

EDTA :

ethylenediamine tetraacetic acid

HEPES :

N-2-hydroxyethylpiperazine-N′2-ethanesulfonic acid

HPLC :

high pressure liquid chromatography

IBMX :

3-isobutyl-1-methylxanthine

JH :

juvenile hormone

ms :

millisecond

nA :

nanoampere

NCA :

I nervi corporis allati I

OCT :

octopamine

TEA :

tetraethyl ammonium

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Authors and Affiliations

  1. Department of Zoology, University of Toronto, M5S 1A1, Toronto, Ontario, Canada

    C. S. Thompson, K. J. Yagi, Z. F. Chen & S. S. Tobe

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  1. C. S. Thompson
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  2. K. J. Yagi
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  3. Z. F. Chen
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  4. S. S. Tobe
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Thompson, C.S., Yagi, K.J., Chen, Z.F. et al. The effects of octopamine on juvenile hormone biosynthesis, electrophysiology, and cAMP content of the corpora allata of the cockroach Diploptera punctata . J Comp Physiol B 160, 241–249 (1990). https://doi.org/10.1007/BF00302589

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