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Competition and persistence of Rhizobium tropici and Rhizobium etli in tropical soil during successive bean (Phaseolus vulgaris L.) cultures

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Abstract

Strains of Rhizobium tropici IIB, CIAT899 and F98.5, both showing good N2 fixation, and a R. etli strain W16.3SB were introduced into a field which had no history of bean culture. Plant dilution estimates showed that in the presence of its host (Phaseolus vulgaris cv. Carioca) during the cropping seasons and the subsequent fallow summer periods, the bean rhizobial populations increased from less than 30 to 103 g−1 dry soil after 1 year and to 104 g−1 dry soil after 2 years. In the 1st year crop, the inoculated strains occupied most of the nodules, which resulted in a higher nodulation and C2H2 reduction activity. Without reinoculation for the second and third crops, however, little R. tropici IIB was recovered from the nodules and the bean population consisted mainly of R. etli, R. leguminosarum bv. phaseoli, and R. tropici IIA. Reinoculation with our superior R. tropici IIB strains before the second crop resulted in R. tropici IIB occupying the main part of the nodules and a positive effect on nodulation and C2H2 reduction activity, but reintroduction of the inoculant strain in the third season did not have any effect.

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Authors and Affiliations

  1. F. A. Janssens Laboratory of Genetics, Catholic University of Leuven, Willem de Croylaan 42, B-3001, Heverlee, Belgium

    K. Vlassak & J. Vanderleyden

  2. CNPAB/EMBRAPA, Itaguaí, Rio de Janeiro, Brazil

    K. Vlassak & A. Franco

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  1. K. Vlassak
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  2. J. Vanderleyden
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  3. A. Franco
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Vlassak, K., Vanderleyden, J. & Franco, A. Competition and persistence of Rhizobium tropici and Rhizobium etli in tropical soil during successive bean (Phaseolus vulgaris L.) cultures. Biol Fertil Soils 21, 61–68 (1996). https://doi.org/10.1007/BF00335994

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