Abstract
Eleven actinomycete melanins were characterized by elemental and functional group and infrared analysis. A soil humic acid from a Brazilian topsoil, a darkred latosol under savanna grassland, analysed previously, was used for comparative purposes. C, N, total acidity, COOH, and phenolic OH contents were within the ranges reported for soil humic acids and fungal melanins. Compared to the soil humic acid, the actinomycete melanins showed greater detail, indicative of higher aliphaticity. Most of these were, in varying degrees, similar to the type III IR spectra of humic acids, which are characteristically high in proteinaceous material, and with variable amounts of polysaccharides. The exceptions were two melanin spectra that showed more resemblance to the humic acid from the dark-red latosol, which belongs to the type I spectra of soil humic acids, a category that includes most soil humic acids. The probable participation of melanic actinomycetes in the formation of humic polymers in discussed.
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References
Becker B, Lechevalier MP, Gordon RE, Lechevalier HA (1964) Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates. Appl Microbiol 12:421–423
Bravard S, Righi D (1991) Characterization of fulvic and humic acids from an Oxisol-Spodosol topsequence of Amazonas, Brazil. Geoderma 48:151–162
Bremner JM (1965) Total nitrogen. In: Black CA, Evans DD, White JL, Ensminger LE, Clark FE (eds) Methods of soil analysis, Part 2. Am Soc Agron, Madison, Wis, pp 1149–1178
Coelho RRR, Linhares LF (1993) Melanogenic actinomycetes (Streptomyces spp.) from Brazilian soils. Biol Fertil Soils 15:220–224
Coelho RRR, Linhares LF, Martin JP (1985) Amino acid distribution in some fungal melanins and soil humic acids from Brazil. Plant and Soil 87:337–346
Coelho RRR, Linhares LF, Martin JP (1988) Sugars in hydrolysates of fungal melanins and soil humic acids. Plant and Soil 106:127–133
Gerazimowicz WY, Byler DM (1985) Carbon 13 CPMA NMR and FTIR spectroscopic studies of humic acids. Soil Sci 39:270–278
Gulyas F (1972) Study of the formation of melanoid pigments by some actinomycetes and microscopic fungi. Symp Biol Hung 11:153–158
Haider K, Martin JP (1970) Humic acid-type phenolic polymers from Aspergillus sidowi culture medium, Stachybotrys spp. cells and autoxidized phenol mixtures. Soil Biol Biochem 2:145–156
Hippe H (1971) Microbial decomposition of radioactive humic acids and melanins. In: Novák, B, Macura J, Kutílek M, Pokorná-Kozová J, Tichý V (eds) Studies about humus. Trans Int Symp Humus et Planta V, Prague, pp 183–189
Huntjens JLM (1972) Amino acid composition of humic-like polymers produced by streptomycetes and of humic acids from pasture and arable land. Soil Biol Biochem 4:339–345
Lechevalier MP, Lechevalier H (1970) Chemical composition as a criterion in the classificaiton of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443
Linhares LF, Martin JP (1978) Decomposition in soil of humic acid-type polymers (melanins) of Eurotium echinulatum, Aspergillus glaucus sp. and other fungi. Soil Sci Soc Am J 42:738–743
Lüdemann HD, Lentz H, Martin JP (1982) Carbon-13 nuclear magnetic resonance spectra of some fungal melanins and humic acids. Soil Sci Soc Am J 46:957–962
Martin JP, Foch DD (1977) Biological properties of soils. In: Elliot LF, Stevenson FJ (eds) Soils for management of organic wastes and waste waters. Am Soc Agron, Madison, Wis, pp 115–169
Martin JP, Haider K (1980) Microbial degradation and stabilization of 14C-labeled lignins, phenols, and phenolic polymers in relation to soil humus formation. In: Kirk TK, Higuchi T, Chang H (eds) Lignin biodegradation: Microbiology, chemistry, and potential applications. CRC Press, Boca Raton, Fla, pp 77–100
Martin JP, Haider K, Saiz Jimenez C (1974) Sodium amalgan reductive degradation of fungal and model phenolic polymers, soil humic acids and simple phenolic compounds. Soil Sci Soc Am Proc 38:760–765
Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M (1980) Thin-layer chromatography of methanolysates of mycolic acidcontaining bacteria. J Chromatogr 188:221–233
Mishra B, Srivastava LL (1990) Physico-chemical characteristics of humic substances of major soil associations of Bihar (India). Plant and Soil 122:185–191
Paim S, Linhares LF, Mangrich AS, Martin JP (1990) Characterization of fungal melanins and soil humic acids by chemical analysis and infrared spectroscopy. Biol Fertil Soils 10:72–76
Schnitzer M (1977) Recent findings on characterization of humic substances extracted from soil from widely differing climatic zones. In: Soil organic matter studies, 2. International Atomic Energy Agency (IAEA), Vienna, pp 117–132
Schnitzer M, Gupta VG (1965) Determination of acidity in soil organic matter. Soil Sci Soc Am Proc 29:274–277
Schnitzer M, Ortiz de Serra MI, Ivarson K (1973) The chemistry of fungal humic acid. Soil Sci Soc Am Proc 37:229–236
Senesi N, Miano TM, Martin JP (1987) Elemental functional infrared and free radical characterization of humic acid-type fungal polymers (melanins). Biol Fertil Soils 5:120–125
Stevenson FJ (1982) Humus chemistry: Genesis, composition, reactions. John Wiley, New York, NY
Stevenson FJ, Goh KM (1971) Infrared spectra of humic acids and related substances. Geochim Cosmochim Acta 35:471–483
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Gomes, R.C., Coelho, R.R.R., Linhares, L.F. et al. Elemental, functional group and infrared spectroscopic analysis of actinomycete melanins from brazilian soils. Biol Fertil Soils 21, 84–88 (1996). https://doi.org/10.1007/BF00335997
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DOI: https://doi.org/10.1007/BF00335997