Abstract
The colonization of Macrophomina phaseolina sclerotia by microbial parasites was evaluated in unsterilized field soil at different levels of soil moisture (0,-5, and-10 kPa) and temperature (20, 30, and 40°C). The maximum colonization of sclerotia was recorded in soil held at-5 or-10 kPa at 30–40°C. Trichoderma harzianum isolate 25–92 and Pseudomonas fluorescens isolate 4–92 were recorded as potential sclerotial parasites, and they significantly (P=0.05) reduced the germination of sclerotia by 60–63%. Cells of P. fluorescens and buffer-washed conidia of T. harzianum were completely agglutinated at 28°C with crude agglutinin of M. phaseolina. The ability of different antagonists to parasitize the sclerotia were correlated with the agglutination ability of the antagonists.
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Srivastava, A.K., Arora, D.K., Gupta, S. et al. Diversity of potential microbial parasites colonizing sclerotia of Macrophomina phaseolina in soil. Biol Fert Soils 22, 136–140 (1996). https://doi.org/10.1007/BF00384445
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DOI: https://doi.org/10.1007/BF00384445