Summary
Degradation of a fungicide, 2,4,5,6-tetrachloroisophthalonitrile (TPN) in soil was studied under laboratory conditions. TPN degraded more rapidly under 60% WHC conditions than at 20%, 40% and 100% WHC, while its degradation was rapid at temperatures of 25°C-30°C, evidently due to the microbial degradation. TPN degraded mainly through dechlorination and partly a substitution reaction. The degradation products identified by gas chromatographic analyses were: 2,4,5-trichloroisophthalonitrile (abbreviated as 2,4,5-Cl3-IPN), 2,4,6-Cl3-IPN, 2,4-Cl2-lPN, 2,5-Cl2-IPN, 4-Cl-IPN, 5-Cl-IPN, IPN, 2,5,6-Cl34-(OH)-IPN and 2,5,6-Cl3-4-(OCH3)-IPN. Peaks with longer retention times than that of TPN were not identified. Tentative degradation pathways were proposed on the basis of the identified degradation products. About 90% of the bacterial strains isolated from the soil to which TPN had been added degraded TPN, suggesting enrichment of the soil with TPN-degrading bacteria.
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Japanese International Cooperation Agency
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Sato, K., Tanaka, H. Degradation and metabolism of a fungicide, 2,4,5,6-tetra-chloroisophthalonitrile (TPN) in soil. Biol Fert Soils 3, 205–209 (1987). https://doi.org/10.1007/BF00640631
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DOI: https://doi.org/10.1007/BF00640631