Summary
The modification of infection pattern in the nervous system and viscera of one hundred and eight mice, which were administered intraperitoneally with herpes simplex virus at various stages of growth ranging from newborn to 30 days old, was investigated with fluorescent antibody method. DD strain mouse was proved to be far more susceptible than C57BL strain mouse.
The lung and liver became less affected in parallel with advance of age, particularly complete absence of infection occurred in the latter organ of even one week old. The gastrointestinal tracts retained high sensitivity through the experiment.
The nervous system displayed a fair susceptibility in all periods of growth. The regions showing definite change of infection were the cerebrum and cerebellum, in which marked but transient increase of infection rate was demonstrated at one week of age. The elevation of infectability in the cerebrum was accompanied by certain topistic vulnerability as seen in the temporal cortex and hippocampus. Peripheries of sympathetic and spinal nerves tended to be more extensively involved after two weeks of age. The spinal cord was representative of lesions with high incidence and stable pattern of infection. No essential change occurred in the range of host cells of the nervous system through the course of aging. The perivascular cellular cuffing took place in the parenchymal lesions of the central nervous system in three weeks old mice. There was no precise accumulation of virus antigen lining the cuffs.
The role and significance of virus pathways, viremia and tissue susceptibility as possible factors taking part in the pattern modification in central nervous system infection were discussed in connection with aging of animals.
Zusammenfassung
Die Modifikation des Infektionsmusters im Zentralnervensystem und in den Körperorganen von 108 Mäusen, die zu verschiedenen Zeiten, vom Neugeborenen bis 30 Tage alt, mit Herpes simplex-Virus intraperitoneal inoculiert worden waren, wurde mit der Fluorescenz-Antikörpermethode untersucht. Der DD-Stamm von Mäusen war viel empfänglicher gegen Herpes simplex-Virus als der C57BL-Stamm.
Die Stärke der Affektion in der Lunge und Leber nimmt parallel mit dem Alter ab. Keine Läsion wurde in der Leber der über 7tägigen Mäuse verursacht. Während der Dauer des Experiments war eine hohe Sensitivität im Gastrointestinaltrakt bemerkbar.
Das Nervensystem zeigte eine mäßig starke Empfänglichkeit in allen Zeiten der Entwicklung. Die Regionen, in denen Veränderungen des Infektionsmusters bestanden, waren Groß- und Kleinhirn, dabei war eine markante, doch vorübergehende Steigerung der Infektionsrate bei 7 tägigen Mäusen auffällig. Die Zunahme der Ansprechbarkeit im Großhirn fiel mit einer topistischen Vulnerabilität des Temporallappens und Hippocampus zusammen. Peripherien der sympathischen und spinalen Nerven tendierten zu stärkerem Befall bei einem Alter über 14 Tage. Im Rückenmark war die Läsion am häufigsten und von stabilem Muster. Durch den Verlauf des Alters gab es keine wesentliche Veränderung der Wirtzellen des ZNS. Die perivasculäre Zellinfiltration fand sich in den parenchymatösen Läsionen des ZNS bei 21 Tage alten Mäusen. Es gab keine deutliche Ansammlung der Virus-Antigene in der direkten Umgebung der Zellinfiltration.
Rolle und Bedeutung gewisser Faktoren, wie der Weg für Viruswanderung, Virämie und Empfänglichkeit des Gewebes, die die Modifikation des Infektionsmusters im ZNS möglicherweise beeinflussen, werden in Beziehung zur Alterung der Tiere erörtert.
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Yamamoto, T., Otani, S. & Shiraki, H. The influence of age on infection in experimental herpes simplex encephalo-myelo-neuritis of mice as demonstrated by fluorescent antibody method. Acta Neuropathol 11, 221–236 (1968). https://doi.org/10.1007/BF00692308
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DOI: https://doi.org/10.1007/BF00692308